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Cross-Species Single-Cell Analysis of Pancreatic Ductal Adenocarcinoma Reveals Antigen-Presenting Cancer-Associated Fibroblasts

UID: 11299

Author(s): Tuveson, David, Robson, Paul, Elyada, Ela, Bolisetty, Mohan, Laise, Pasquale, Flynn, William F., Courtois, Elise T., Burkhart, Richard A., Teinor, Jonathan A., Belleau, Pascal, Biffi, Giulia, Lucito, Matthew, Sivajothi, Santhosh, Armstrong, Todd D., Engle, Dannielle, Yu, Kenneth H.*, Hao, Yuan, Wolfgang, Christopher L., Park, Youngkyu, Preall, Jonathan, Jaffee, Elizabeth M., Califano, Andrea * MSK affiliated

Description
Description from dbGaP:

"Cancer-associated fibroblasts (CAFs) are major players in the progression and drug resistance of pancreatic ductal adenocarcinoma (PDAC). CAFs constitute a diverse cell population consisting of several recently described subtypes, although the extent of CAF heterogeneity has remained undefined. Here we employ single-cell RNA-sequencing to thoroughly characterize the neoplastic and tumor microenvironment content of human PDAC tumors. Six human PDAC tumor specimens from six patients were collected, and processed for single-cell RNA-sequencing analysis. Adjacent-normal pancreas tissue was also collected from two of the patients. Tumor samples were digested, and fluorescence-activated cell sorting was used to isolate viable cells. For one tumor sample, viable, CD45-negative, CD31-negative, and EpCAM-negative cells were also isolated to enrich for CAFs. The 10X Chromium platform was then used to isolate single cells for RNA-sequencing analysis. This work has demonstrated the differences in immune cell populations between adjacent-normal and tumor tissues, and identified subpopulations of epithelial cells and CAFs present in PDAC tumors. This high-throughput analysis is a resource to better understand the cell populations present in PDAC, and may ultimately aid in the development of more effective therapies for this deadly malignancy."
Subject(s)
Cancer-Associated Fibroblasts
Carcinoma, Pancreatic Ductal
Epithelial Cells
RNA-Seq
OncoTree Cancer Type(s)
Pancreatic Adenocarcinoma
Access via dbGaP


Accession #: phs001840.v1.p1

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Associated Publications
Elyada E, Bolisetty M, Laise P, Flynn W, Courtois E, Burkhart R, Teinor J, Belleau P, Biffi G, Lucito M, Sivajothi S, Armstrong T, Engle D, Yu K, Hao Y, Wolfgang C, Park Y, Preall J, Jaffee E, Califano A, Robson P, Tuveson D. Cross-species single-cell analysis of pancreatic ductal adenocarcinoma reveals antigen-presenting cancer-associated fibroblasts. Cancer Discovery. 2019; 9(8):1102-1123.
Equipment Used
Illumina HiSeq 4000

High throughput gene sequencing system

Dataset Format(s)
PDF, XML, gzip, TXT
Dataset Size
293.53 MB
Data Catalog Record Updated
2024-07-11