CRISPR enhancer scan to detect critical elements for the regulation of EVI1 in a translocated enhancer

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UID: 11326

Author(s): Delwel, Ruud

Description: Description from EMBL-EBI:

"To uncover, in an unbiased fashion, which elements of the 18 kb translocated region control EVI1 transcription, we devised a CRISPR/Cas9-based enhancer scanning approach. We considered all possible sgRNA target sites containing a canonical Cas9 PAM site (NGG) on both strands of the minimal 18 kb translocated region. Deep-sequencing libraries were generated by PCR amplification of sgRNA guide strands using primers that tag the product with standard Illumina adapters and a 4 bp sample barcode in a 2 step-PCR protocol."

Detailed sample and protocol information via EMBL-EBI.

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