KO_Vehicle_H3K27ac

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UID: 11334

Description: Extraction protocol from GEO:

"Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification.
DNA fragments range from 200-600 bp were size-selected for subsequent ChIP-seq library preparation with TruSeq ChIP Sample Prep Kit (Illumina) according to manufacturer’s instructions. Sequencing libraries were multiplexed and ran on a Hiseq2500 platform"

Subject of Study

Subject(s)

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