Bulk RNA-Sequencing of mouse large intestinal lymphatics (capillaries and collectors)

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UID: 11341

Author(s): Niec, Rachel E.*, Chu, Tin Yi*, Gur-Cohen, Shiri, Schernthanner, Marina, Pe'er, Dana*, Fuchs, Elaine * MSK affiliated

Description: Summary from GEO:

"Purpose: Profile global gene expression of intestinal lymphatics to compare with published datasets
Methods: Intestinal tissues were digested, lymphatic endothelial cells were sorted using lymphatic markers including CD31, Podoplanin, Lyve1. Total RNA was isolated from sorted cells using the Direct-Zol RNA MiniPrep kit (Zymo). Samples were submitted for bulk RNA-Sequencing.
Conclusions: Gobal gene expression profile of intestinal lymphatics for further comparison with published datasets"

Overall design from GEO:

"Intestinal tissues were digested, lymphatic endothelial cells were sorted using lymphatic markers including CD31, Podoplanin, Lyve1. Total RNA was isolated from sorted cells using the Direct-Zol RNA MiniPrep kit (Zymo). Samples were submitted for bulk RNA-Sequencing."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE199082

Access via BioProject

Accession #: PRJNA818309
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus and BioProject databases provides open access to these files.
Associated Publications: Niec R, Chu T, Schernthanner M, Gur-Cohen S, Hidalgo L, Pasolli H, Luckett K, Wang Z, Bhalla S, Cambuli F, Kataru R, Ganesh K, Mehrara B, Pe'er D, Fuchs E. Lymphatics act as a signaling hub to regulate intestinal stem cell activity. Cell Stem Cell. 2022; 29(7):1067-1082.e18.
Equipment Used: Illumina NovaSeq 6000
Dataset Format(s): CSV, TAR
Dataset Size: 940.0 KB

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