RNA profiling of human lymphatic endothelial cells exposed to melanoma exosomes

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UID: 11344

Author(s): García-Silva, Susana, Graña-Castro, Osvaldo, Peinado, Héctor

Description: Summary from GEO:

"In order to study the influence of melanoma-derived exosomes in the lymphatic vasculature within the lymph nodes, we have profile the transcriptional changes occurring in human lymphatic endothelial cells treated with melanoma-secreted exosomes or with the conditioned medium from melanoma cells depleted of exosomes."

Overall design from GEO:

"Human lymphatic endothelial cells (HMVECs) were treated for 24h and 48h with exosomes from SK-MEL-147 melanoma cell line and conditioned medium from the same line depleted of exosomes. RNA was extracted with trizol and subsequent Qiagen columns. RNA sequencing was performed afterwards."

Subject of Study

Subject(s)

OncoTree Cancer Type(s)

Melanoma

Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, BioProject, and SRA databases provide open access to these files.
DOI: 10.1038/s43018-021-00272-y
Associated Publications: García-Silva S, Benito-Martín A, Nogués L, et al. Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism. Nature Cancer. 2021; 2(12):1387-1405.
Equipment Used: Illumina HiSeq 1500
high-throughput sequencing
Dataset Format(s): TXT
Dataset Size: 1 MB

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