MEF to induced endoderm progenitors reprogramming

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UID: 11411

Author(s): Lange, Marius, Klein, Michal

Description: Description from Figshare:

"scRNA-seq time-series dataset of mouse embryonic fibroblast (MEF) reprogramming to induced endoderm progenitors (iEPs)
assayed using Dropseq and 10x Genomics. Contains 8 time points spanning days 0-28 during reprogramming. Total cell number is 104,887.

This dataset contains raw spliced and unspliced counts estimated using the velocyto pipeline (La Manno et al., Nature 2018) that can be used to compute RNA velocity. Further, this dataset contains clonal information from CellTagging.

Please cite the original publication: https://doi.org/10.1038/s41586-018-0744-4"

Subject of Study

Subject(s)

Access via figshare
Access Restrictions: Free to All
Access Instructions: Available for download via Figshare
DOI: 10.6084/m9.figshare.c.5172299.v2
Associated Publications: Lange M, Bergen V, Klein M, Setty M, Reuter B, Bakhti M, Lickert H, Ansari M, Schniering J, Schiller H, Pe’er D, Theis F. CellRank for directed single-cell fate mapping. Nature Methods. 2022; 19(2):159-170.
Equipment Used: 10X Chromium
10x Genomics' Chromium technology partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called GEMs (Gel Bead-In EMulsions). This core technology can be used to partition single cells, nuclei, or high molecular weight gDNA to prepare next generation sequencing libraries in parallel.

Dropseq
enables biologists to analyze RNA expression genome-wide in thousands of individual cells at onc
Dataset Format(s): H5AD
Dataset Size: 1.48 GB

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