Pancreas development preprocessed

UID: 11414

Description: Description from Figshare:

"Preprocessed scRNA-seq dataset of pancreatic development at E15.5 in mice assayed using 10x genomics. Total cell number is 2531. Contains RNA velocity (La Manno et al., Nature 2018) computed using scVelo (Bergen et al., Nature Biotechnology 2020).

Please cite the original publication: https://doi.org/10.1242/dev.173849"

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DOI: 10.6084/m9.figshare.c.5172299
Associated Publications: Lange M, Bergen V, Klein M, Setty M, Reuter B, Bakhti M, Lickert H, Ansari M, Schniering J, Schiller H, Pe’er D, Theis F. CellRank for directed single-cell fate mapping. Nature Methods. 2022; 19(2):159-170.
Equipment Used: 10X Chromium
10x Genomics' Chromium technology partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called GEMs (Gel Bead-In EMulsions). This core technology can be used to partition single cells, nuclei, or high molecular weight gDNA to prepare next generation sequencing libraries in parallel.
Software Used: scVelo
RNA velocity generalized through dynamical modeling
Dataset Format(s): H5AD
Dataset Size: 140.16 MB