Pancreas development

UID: 11415

Description: Description from Figshare:

"scRNA-seq dataset of pancreatic development at E15.5 in mice assayed using 10x genomics. Total cell number is 2531.

This dataset contains raw spliced and unspliced counts estimated using the velocyto pipeline (La Manno et al., Nature 2018) that can be used to compute RNA velocity.

Please cite the original publication: https://doi.org/10.1242/dev.173849"

Subject of Study

Subject(s)

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Access Restrictions: Free to All
Access Instructions: Download from Figshare to access
DOI: 10.6084/m9.figshare.c.5172299
Associated Publications: Lange M, Bergen V, Klein M, Setty M, Reuter B, Bakhti M, Lickert H, Ansari M, Schniering J, Schiller H, Pe’er D, Theis F. CellRank for directed single-cell fate mapping. Nature Methods. 2022; 19(2):159-170.
Equipment Used: 10X Chromium
10x Genomics' Chromium technology partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called GEMs (Gel Bead-In EMulsions). This core technology can be used to partition single cells, nuclei, or high molecular weight gDNA to prepare next generation sequencing libraries in parallel.
Software Used: Velocyto
A library for the analysis of RNA velocity
Dataset Format(s): H5AD
Dataset Size: 33.52 MB