Directed differentiation of oligodendrocytes from human embryonic stem cells

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UID: 11422

Author(s): Major, Tamara*, Tabar, Viviane S* * MSK affiliated

Description: Summary from GEO:

"Here we compare the transcriptional profile of the undifferentiated hESC with the anterior oligodendroctes derived from the hESC using our new differentiation protocol."

Overall design from GEO:

"Total RNA was isolated from undifferentiated hES cells and from hES derived oligodendrocytes, 70 days old and FACS sorted for O4 marker. Samples for each group in triplicate were processed for Illumina bead arrays (Illumina HT-12) by the MSKCC genomics core facility according to the specifications of the manufacturer."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE63318

Access via BioProject

Accession #: PRJNA267445
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus and BioProject databases provides open access to these files.
Associated Publications: Piao J, Major T, Auyeung G, Policarpio E, Menon J, Droms L, Gutin P, Uryu K, Tchieu J, Soulet D, Tabar V. Human embryonic stem cell-derived oligodendrocyte progenitors remyelinate the brain and rescue behavioral deficits following radiation. Cell Stem Cell. 2015; 16(2):198-210.
Equipment Used: Illumina HumanHT-12 V4.0 expression beadchip
Dataset Format(s): TAR, TXT
Dataset Size: 29.6 MB

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