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The dataset for Genome-wide cell-free DNA fragmentation in patients with cancer
- Description
Description from EGA: "The dataset for Genome-wide cell-free DNA fragmentation in patients with cancer includes 538 bam files from whole genome next-generation sequencing on the Illumina HiSeq2500. The samples analyzed include plasma samples from healthy individuals and patients with cancer."
- Subject
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DNA FragmentationPlasmaWhole Genome Sequencing
- Access Rights
- Application Required
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WT_Vehicle_H3K27me3
- Description
Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....
- Subject
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Cell Culture TechniquesChromatin Immunoprecipitation SequencingDNA FragmentationHistones
- Access Rights
- Free to All
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WT_Vehicle_H3K27ac
- Description
Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....
- Subject
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Cell Culture TechniquesChromatin Immunoprecipitation SequencingDNA FragmentationHistones
- Access Rights
- Free to All