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  • The dataset for Genome-wide cell-free DNA fragmentation in patients with cancer

    Description

    Description from EGA: "The dataset for Genome-wide cell-free DNA fragmentation in patients with cancer includes 538 bam files from whole genome next-generation sequencing on the Illumina HiSeq2500. The samples analyzed include plasma samples from healthy individuals and patients with cancer."

    Subject
    DNA Fragmentation
    Plasma
    Whole Genome Sequencing
    Access Rights
    Application Required
  • WT_Vehicle_H3K27me3

    Description

    Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....

    Subject
    Cell Culture Techniques
    Chromatin Immunoprecipitation Sequencing
    DNA Fragmentation
    Histones
    Access Rights
    Free to All
  • WT_Vehicle_H3K27ac

    Description

    Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....

    Subject
    Cell Culture Techniques
    Chromatin Immunoprecipitation Sequencing
    DNA Fragmentation
    Histones
    Access Rights
    Free to All