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Pull-down of Mlh3-Flag from yeast meiotic cells and analysis of pulled-down partner proteins
- Authors
- Loew, DamarysSabatet, Valentin
- Description
Description from Proteome Xchange: "Mlh3 internally tagged with Flag was pulled down from synchronous meiotic cells at t = 4 h in meiosis, in order to identify its interacting partners involved in meiotic crossover formation" Sample Processing Protocol from PRIDE: "2.1010 cells of VBD1674 strain at 4 h in meiosis were harvested, washed two times with ice-cold TNG buffer (50 mM Tris/HCl pH 8; 150...
- Subject
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MeiosisMutL ProteinsProteomeRecombination, Genetic
- Access Rights
- Free to All
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Mechanism of in vivo activation of the MutLgamma-Exo1 complex for meiotic crossover formation
- Authors
- Borde, Valerie
- Description
Summary from the GEO: "Programmed DNA double-strand breaks (DSBs) initiate meiotic recombination and their subsequent repair culminates in crossover (CO) formation. COs result from the asymmetric cleavage of double-Holliday junction (dHJ) intermediates, that requires the MutLγ complex together with a non-catalytic function of Exo1, an activity essential for fertility but at risk of generating unwanted...
- Subject
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DNA Breaks, Double-StrandedMeiosisMutL ProteinsProtein BindingRecombination, Genetic
- Access Rights
- Free to All
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Mechanism of in vivo activation of the MutL-Exo1 complex for meiotic crossover formation
- Authors
- Sanchez, AuroreMu, XiaojingBorde, ValerieKeeney, Scott
- Description
Summary from the GEO: "Programmed DNA double-strand breaks (DSBs) initiate meiotic recombination and their subsequent repair culminates in crossover (CO) formation. COs result from the asymmetric cleavage of double-Holliday junction (dHJ) intermediates, that requires the MutLγ endonuclease and a non-catalytic function of Exo1, an activity essential for fertility but at risk of generating unwanted...
- Subject
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DNA Breaks, Double-StrandedMeiosisMutL ProteinsProtein BindingRecombination, Genetic
- Access Rights
- Free to All
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Pull-down of Exo1-TAP from yeast meiotic cells and analysis of phosphorylated residues
- Authors
- Loew, DamarysSabatet, Valentin
- Description
Description from the ProteomeXchange: "Exo1 tagged with the TAP sequence was pulled down from synchronous ndt80∆ meiotic cells after induction of the CDC5 kinase at t = 8 h 15 in meiosis (1 h 15 after CDC5 induction), in order to identify its phosphorylated residues" PRIDE Sample Processing Protocol: "2.1010 cells of VBD2070 strain were harvested after 8 h 15 min in sporulation medium and processed...
- Subject
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MeiosisMutL ProteinsProteomeRecombination, Genetic
- Access Rights
- Free to All