A chemical strategy for protease substrate profiling

Authors

Griswold, Andrew

Bachovchin, Daniel

Description

Here, we introduce a simple method, termed CHOPS, for the discovery of protease substrates. CHOPS exploits a 2-pyridinecarboxaldehyde (2PCA)-biotin probe, which selectively biotinylates protein N-termini except those with proline in the second position. CHOPS can, in theory, discover substrates for any protease, but is particularly well-suited to discover canonical DPP substrates, as cleaved but not...

Subject

Biotin

Leukemia

Mass spectrometry

Peptide Hydrolases

Substrate Specificity

Access Rights

Free to All