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Flura-Seq Identifies In Situ Transcriptomes of Micrometastases
- Authors
- Basnet, HariharMacalinao, Danilo Reynaldo GalindezMassagué, Joan
- Description
Summary from the GEO: "Understanding the biology of rare cell populations in the context of their microenvironment requires an accurate analysis of the transcriptomes of these cells as expressed in situ. We developed fluorouracil-tagged RNA sequencing (Flura-seq) to characterize the transcriptomes of small cell subpopulations from a whole organ in model systems. The method utilizes cytosine deaminase...
- Subject
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Brain NeoplasmsBreast NeoplasmsGene Expression ProfilingLung NeoplasmsTranscriptomeTransforming Growth Factor beta
- Access Rights
- Free to All
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Transforming Growth Factor-b Suppresses Type 2 Immunity to Cancer
- Authors
- Liu, MingKuo, FengshenLi, Ming O.
- Description
Summary from GEO: "The immune system employs two distinct defense strategies against infections: pathogen elimination typified by type 1 immunity, and pathogen containment exemplified by type 2 immunity concomitant with tissue repair. Akin to infectious diseases, cancer progresses with cancer cell acquisition of microorganism-like behavior propagating at the expense of the host. While immunological...
- Subject
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CD4-Positive T-Lymphocytesinterleukin-4Th2 CellsTransforming Growth Factor beta
- Access Rights
- Free to All
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ID1 mediates escape from TGFβ Tumor Suppression in Pancreatic Cancer
- Authors
- Huang, Yun-HanMassagué, Joan
- Description
Summary from GEO: "We perform ChIPseq, ATACseq, and RNAseq to characterize the role of ID1 in PDA cells." Overall design from GEO: "ChIPseq, RNAseq, and ATACseq are used to profile PDA cells"
- Subject
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Carcinoma, Pancreatic DuctalChromatin Immunoprecipitation SequencingInhibitor of Differentiation Protein 1Pancreatic NeoplasmsRNA-SeqTransforming Growth Factor beta
- Access Rights
- Free to All