Search Tips
of 1 Next >
Results Found: 7
  • AICDA-induced epigenetic plasticity accelerates germinal center-derived lymphomagenesis

    Authors
    Teater, Matt
    Elemento, Olivier
    Melnick, Ari M.
    Description

    Summary from the GEO: "Epigenetic heterogeneity is emerging as a significant phenotypic feature of tumors. In diffuse large B-cell lymphomas (DLBCLs), increased cytosine methylation heterogeneity is associated with poor clinical outcome, yet the biological mechanisms driving epigenetic heterogeneity remain unclear. Activation-induced cytidine deaminase (AICDA), an enzyme that deaminates and facilitates...

    Subject
    Cytidine Deaminase
    Germinal Center
    Lymphoma, Large B-Cell, Diffuse
    Access Rights
    Free to All
  • DNA Methylation Dynamics of Germinal Center B Cells Are Mediated by AID

    Authors
    Teater, Matt
    Elemento, Olivier
    Description

    Summary from the GEO: "Changes in DNA methylation are required for the formation of germinal centers (GC), but the mechanisms of such changes are poorly understood. Activation-induced cytidine deaminase (AID) has been recently implicated recently in DNA demethylation through its deaminase activity coupled with DNA repair. We investigated the epigenetic function of AID in vivo in germinal center B...

    Subject
    B-Lymphocytes
    Cytidine Deaminase
    Germinal Center
    Somatic Hypermutation, Immunoglobulin
    Access Rights
    Free to All
  • Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors [ABE-max]

    Authors
    Grunewald, Julian
    Lareau, Caleb A.
    Description

    Summary from GEO: "CRISPR-Cas base editor technology enables targeted nucleotide alterations and is being rapidly deployed for research and potential therapeutic applications. The most widely used base editors induce DNA cytosine (C) deamination with rat APOBEC1 (rAPOBEC1) enzyme, which is targeted by a linked Cas protein-guide RNA (gRNA) complex. Previous studies of cytosine base editor (CBE) specificity...

    Subject
    APOBEC-1 Deaminase
    CRISPR-Cas Systems
    Cytidine Deaminase
    Cytosine
    Gene Editing
    Access Rights
    Free to All
  • Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors [Modifications - validation]

    Authors
    Grunewald, Julian
    Lareau, Caleb A.
    Description

    Summary from GEO: "Base Editing has been touted the most intelligent and precise application of the CRISPR platform so far, merging the simplicity of RNA-guided nucleases with deaminases that allow for the programmable generation of single base substitutions - without introduction of double-strand breaks. Even though the two-component system has been expected to cause off-target substitutions, studies...

    Subject
    APOBEC-1 Deaminase
    CRISPR-Cas Systems
    Cytidine Deaminase
    Cytosine
    Gene Editing
    Access Rights
    Free to All
  • Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors [P2A-EGFP control]

    Authors
    Grunewald, Julian
    Lareau, Caleb A.
    Description

    Summary from GEO: "Base Editing has been touted the most intelligent and precise application of the CRISPR platform so far, merging the simplicity of RNA-guided nucleases with deaminases that allow for the programmable generation of single base substitutions - without introduction of double-strand breaks. Even though the two-component system has been expected to cause off-target substitutions, studies...

    Subject
    APOBEC-1 Deaminase
    CRISPR-Cas Systems
    Cytidine Deaminase
    Cytosine
    Gene Editing
    RNA-Seq
    Access Rights
    Free to All
  • Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors [Modifications - screen]

    Authors
    Grunewald, Julian
    Lareau, Caleb A.
    Description

    Summary from GEO: "Base Editing has been touted the most intelligent and precise application of the CRISPR platform so far, merging the simplicity of RNA-guided nucleases with deaminases that allow for the programmable generation of single base substitutions - without introduction of double-strand breaks. Even though the two-component system has been expected to cause off-target substitutions, studies...

    Subject
    APOBEC-1 Deaminase
    CRISPR-Cas Systems
    Cytidine Deaminase
    Cytosine
    Gene Editing
    Access Rights
    Free to All
  • Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors [BaseEditors - RNA]

    Authors
    Grunewald, Julian
    Lareau, Caleb A.
    Description

    Summary from GEO: "Base Editing has been touted the most intelligent and precise application of the CRISPR platform so far, merging the simplicity of RNA-guided nucleases with deaminases that allow for the programmable generation of single base substitutions - without introduction of double-strand breaks. Even though the two-component system has been expected to cause off-target substitutions, studies...

    Subject
    APOBEC-1 Deaminase
    CRISPR-Cas Systems
    Cytidine Deaminase
    Cytosine
    Gene Editing