Inhibition of MYC by the SMARCB1 tumor suppressor
UID: 10605
- Description
- Summary from the GEO: "We report that the protein encoded by the SMARCB1 gene, SNF5, is capable of inhibiting MYC binding in vitro and in a malignant rhabdoid tumor (MRT) cell line. By comparing the effects of reintroduction of SNF5 with genetic inhibition of MYC (OMOMYC) on multiple aspects of chromatin remodeling and transcription in MRT cells, we show that regulation of MYC binding by SNF5 is not connected to the role of SNF5 in chromatin remodeling, but instead is responsible for controlling RNA polymerase pause release during transcription. Our data reveal that SNF5 negatively regulates MYC function and may explain how loss of SNF5 can lead to rapid tumorigenesis."
Overall design from the GEO: "The G401 MRT cell line was transduced with lentiviral vectors expressing tetracycline (TET)-inducible version of EGFP, SNF5, or OMOMYC. Immediately following selection, each group of transduced cells were treated with 1ug/ml doxycycline for 24 hours. All ChIP-seq, ATAC-seq, and PRO-Seq experiments were performed at the 24 hour timepoint."
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Access via GEO
Narrowpeak and Plain Text Data of expression profiling by high throughput sequencing
Accession #: GSE109310Access via SRAPRO, ATAC and ChIP sequencing for 30 samples
Accession #: SRP131080Access via BioProjectAdditional information about overall initiative.
Accession #: PRJNA430400 - Access Restrictions
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Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus, SRA and BioProject databases provide open access to these files.
- Associated Publications
- Data Type
- Equipment Used
- Software Used
- Dataset Format(s)
- Plain Text, SRA, TAR, gzip, NARROWPEAK
- Dataset Size
- 1.6 MB (TXT), 4.4MB (TAR of NARROWPEAK), 95.6 (SRA)
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