Whole genome ChIP-seq of histone H3 threonine 11 phosphorylation in Saccharomyces cerevisiae

UID: 10670

Author(s): Murakami, Hajime, Kniewel, Ryan*, Keeney, Scott * MSK affiliated

Description: Summary from the GEO: "We used ChIP-seq to determine the whole-genome enrichment of histone H3 threonine 11 phosphorylation (H3 T11ph) during Saccharomyces cerevisiae meiosis. S. cerevisiae SK1 cells were synchronized for meiotic entry and 3 and 4 hour meiotic samples were obtained. As H3 T11ph is dependent on the formation of meiotic double strand breaks (DSBs), a negative control ChIP-seq sample was obtained from a strain lacking DSBs (spo11-yf). Concurrently, ChIP-seq was carried out for histone H3 as a control for comparision.

ChIP-seq samples total were taken from the following conditions: Anti-H3 T11ph and anti-H3, 3 and 4 hours synchronous meiosis, biological replicates from wild type strain (8 samples). Anti-H3 T11ph and anti-H3, 3.5 hours synchronous meiosis single sample from spo11-Y135F DSB-negative mutant (2 samples)."

Subject of Study

Subject(s)

Access via GEO
Raw and TAR sequencing data.
Accession #: GSE100564

Access via SRA
ChIP-Seq reads for 10 samples.
Accession #: SRP110610

Access via BioProject
Additional information about overall initiative.
Accession #: PRJNA392121
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
Associated Publications: Kniewel R, Murakami H, Liu Y, Ito M, Ohta K, Hollingsworth N, Keeney S. Histone H3 threonine 11 phosphorylation is catalyzed directly by the meiosis-specific kinase Mek1 and provides a molecular readout of Mek1 activity in vivo. Genetics. 2017; 207(4):1313-1333.
Data Type: Genomic
Equipment Used: Illumina HiSeq 2000
High throughput gene sequencing system
Dataset Format(s): Plain Text, TAR, RAW
Dataset Size: 662.7 Mb (TAR of TXT), 7.62 Gb (SRA)

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