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Whole genome ChIP-seq of histone H3 threonine 11 phosphorylation in Saccharomyces cerevisiae

UID: 10670

Author(s): Murakami, Hajime, Kniewel, Ryan*, Keeney, Scott * MSK affiliated

Description
Summary from the GEO: "We used ChIP-seq to determine the whole-genome enrichment of histone H3 threonine 11 phosphorylation (H3 T11ph) during Saccharomyces cerevisiae meiosis. S. cerevisiae SK1 cells were synchronized for meiotic entry and 3 and 4 hour meiotic samples were obtained. As H3 T11ph is dependent on the formation of meiotic double strand breaks (DSBs), a negative control ChIP-seq sample was obtained from a strain lacking DSBs (spo11-yf). Concurrently, ChIP-seq was carried out for histone H3 as a control for comparision.

ChIP-seq samples total were taken from the following conditions: Anti-H3 T11ph and anti-H3, 3 and 4 hours synchronous meiosis, biological replicates from wild type strain (8 samples). Anti-H3 T11ph and anti-H3, 3.5 hours synchronous meiosis single sample from spo11-Y135F DSB-negative mutant (2 samples)."
Subject of Study
Subject(s)
Access via GEO

Raw and TAR sequencing data.
Accession #: GSE100564

Access via SRA

ChIP-Seq reads for 10 samples.
Accession #: SRP110610

Access via BioProject

Additional information about overall initiative.
Accession #: PRJNA392121

Access Restrictions
Free to All
Access Instructions
The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
Associated Publications
Data Type
Equipment Used
Illumina HiSeq 2000
Dataset Format(s)
Plain Text, TAR, RAW
Dataset Size
662.7 Mb (TAR of TXT), 7.62 Gb (SRA)
Data Catalog Record Updated
2023-12-07