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Results Found: 5
  • An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression

    Authors
    Albanese, Steven Kyle
    Parton, Daniel Lawrence
    Isik, Mehtap
    Rodriguez-Laureano, Lucelenie
    7 more author(s)...
    Description

    Source code, assay data, full expression results for all ABL1 constructs, wild-type kinases, and ABL1 and SRC mutants, plasmid libraries, and other supporting material for the generation of a library of human kinase domain constructs for automated bacterial expression. From the publication abstract, "Kinases play a critical role in cellular signaling and are dysregulated in a number of diseases,...

    Subject
    Bacteria/metabolism
    Binding sites
    Escherichia coli/metabolism
    Phosphorylation
    Protein Structure, Secondary
    Protein-Tyrosine Kinases
    Proto-Oncogene Proteins c-abl
    src-Family Kinases
    Access Rights
    Free to All
    Fee Required
  • Histone H3 threonine 11 phosphorylation is catalyzed directly by the meiosis-specific kinase Mek1 and provides a molecular readout of Mek1 activity in vivo

    Authors
    Kniewel, Ryan
    Murakami, Hajime
    Liu, Yan
    Ito, Masaru
    3 more author(s)...
    Description

    Summary from the GEO: "Saccharomyces cerevisiae Mek1 is a CHK2/Rad53-family kinase that regulates meiotic recombination and progression upon its activation in response to DNA double-strand breaks (DSBs). The full catalog of direct Mek1 phosphorylation targets remains unknown. Here, we show that phosphorylation of histone H3 on threonine 11 (H3 T11ph) is induced by meiotic DSBs in S. cerevisiae and...

    Subject
    Checkpoint Kinase 2
    Chromatin Immunoprecipitation Sequencing
    Histones
    Phosphorylation
    Protein-Serine-Threonine Kinases
    Access Rights
    Free to All
  • Whole genome ChIP-seq of histone H3 threonine 11 phosphorylation in Saccharomyces cerevisiae

    Authors
    Murakami, Hajime
    Kniewel, Ryan
    Keeney, Scott
    Description

    Summary from the GEO: "We used ChIP-seq to determine the whole-genome enrichment of histone H3 threonine 11 phosphorylation (H3 T11ph) during Saccharomyces cerevisiae meiosis. S. cerevisiae SK1 cells were synchronized for meiotic entry and 3 and 4 hour meiotic samples were obtained. As H3 T11ph is dependent on the formation of meiotic double strand breaks (DSBs), a negative control ChIP-seq sample...

    Subject
    Chromatin Immunoprecipitation Sequencing
    Histones
    Phosphorylation
    Protein-Serine-Threonine Kinases
    Access Rights
    Free to All
  • PI3K inhibition activates SGK1 via a feedback loop to promote chromatin-based regulation of ER-dependent gene expression

    Authors
    Toska, Eneda
    Koche, Richard Patrick
    Description

    Summary from GEO: "The phosphoinositide 3-kinase (PI3K) pathway integrates extracellular stimuli to phosphorylate and activate key downstream effectors such as AKT and serum-and glucocorticoid-inducible kinase (SGK1). We have previously reported that the PI3K pathway regulates ER-dependent transcription in breast cancer through the phosphorylation of the epigenetic regulator KMT2D by AKT. Here, we...

    Subject
    Chromatin Assembly and Disassembly
    Phosphatidylinositol 3-Kinase
    Phosphoinositide-3 Kinase Inhibitors
    Phosphorylation
    Receptors, Estrogen
    Access Rights
    Free to All
  • Cell type-dependent differential activation of ERK by oncogenic KRAS in human colon cancer and mouse intestinal epithelium

    Authors
    Brandt, Raphael
    Sell, Thomas
    Lüthen, Mareen
    Uhlitz, Florian
    13 more author(s)...
    Description

    Summary from GEO: "Mutations activating the KRAS GTPase or the BRAF kinase are frequent in colorectal cancer and are thought to constitutively activate the terminal mitogen-activated protein kinase, ERK. Using mass cytometry, we found graded phosphorylation of ERK anti-correlated with cell differentiation in patient-derived colorectal cancer organoids, and unexpectedly this gradient was observed...

    Subject
    Cell Differentiation
    Colonic Neoplasms
    Genes, ras
    Mitogen-Activated Protein Kinases
    Phosphorylation
    Proto-Oncogene Proteins B-raf
    RNA-Seq
    Single-Cell Analysis
    Access Rights
    Free to All