Host immunity modulates efficacy of microbiota transplantation in treatment of Clostridioides difficile infection

UID: 10781

Publisher(s): University of Chicago

Description: Summary from BioProject: "Rag1 heterozygote mice and Rag1 knockout mice are infected with C.diff and given FMTs to clear infection to demonstrate the role of the immune system in C.diff infection clearance"

Subject of Study

Subject(s)

Access via BioProject
Amplicon sequencing of 123 samples
Accession #: PRJNA668190
Access Restrictions: Free to All
Access Instructions: The NCBI BioProject and SRA databases provide open access to these files.
Associated Publications: Littmann E, Lee J, Denny J, et al. Host immunity modulates the efficacy of microbiota transplantation for treatment of Clostridioides difficile infection. Nature Communications. 2021; 12:755.
Data Type: Genomic
Equipment Used: Illumina MiSeq
The MiSeq is an integrated instrument that performs clonal amplification, genomic DNA sequencing, and data analysis with base calling, alignment, variant calling, and reporting in a single run.
Software Used: SRA Toolkit
The NCBI SRA Toolkit enables reading ("dumping") of sequencing files from the SRA database and writing ("loading") files into the .sra format.
Dataset Format(s): FASTQ
Data Tool(s): Amplicon Sequencing
Dataset Size: 2 GB