High-throughput JH4 sequencing of germinal centers of LDHA-suffiicient and -deficient B cells
Alternate Titles(s): Distinct Metabolic Requirements Regulate B Cell Activation and Germinal Center Responses
UID: 10906
- Description
- Summary from GEO:
"Following infection or vaccination, activated B cells at extrafollicular sites or within germinal centers (GCs) undergo vigorous clonal proliferation. Proliferating lymphocytes have been shown to undertake lactate dehydrogenase A (LDHA)-dependent aerobic glycolysis; however, the specific role of this metabolic pathway in a B cell transitioning from a naïve to a highly proliferative, activated state remains poorly defined. Here, we deleted LDHA in a stage- and cell-specific manner. We find that ablation of LDHA in a naïve B cell did not profoundly affect its ability to undergo a T cell-independent extrafollicular B cell response. On the other hand, LDHA-deleted naïve B cells had a severe defect in the capacities to form GCs and mount GC-dependent antibody responses. In addition, loss of LDHA in T cells severely compromised B cell-dependent immune responses. Strikingly, when LDHA was deleted in activated, as opposed to naïve, B cells, there were only minimal effects on the GC reaction and in the generation of high-affinity antibodies. These findings strongly suggest that naïve and activated B cells have distinct metabolic requirements that are further regulated by niche and cellular interactions."
Overall design from GEO:
"JH4 sequencing of LDHA-sufficient and -deficient splenocytes"
-
Access via GEO
Accession #: GSE232658Access via BioProject
Accession #: PRJNA973112 - Access Restrictions
-
Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus and BioProject databases provides open access to these files.
- Associated Publications
- Equipment Used
-
Illumina NovaSeq 6000
- Dataset Format(s)
- CSV, TAR, TXT
- Dataset Size
- 3.1 MB (TAR), 3.6 MB (CSV)
Do you have or know of a dataset that should be added to the catalog? Let us know!