Summary from the GEO: "Complete Amplicon Sequencing of a 500bp V(D)J recombination products from proB cells sorted from control and Setd2KO mice to Vh558, Vh7183 and Vhq52 variable gene familes to JH4 joining gene fragment to determine CDR3. Genomic DNA from proB cells (B220+Cd43+IgM-CD25-) sorted from control and Setd2 knockout mice were isolated and subjected to V(D)J recombination PCR using a primer...

Summary from the GEO: "We study the role of loss of Setd2 and H3K36me3 in early B cell development through NGS methods including RNA, ChIP and ATAC Seq of sorted B cell populations of control and Setd2KO primary B cells from mice. From age-matched littermate controls and Setd2KO mouse, we sorted early proB cells (B220+CD43+CD19-Cd25-IgM-) and performed ATAC-seq, RNA-Seq, and ChIP-seq using an antibody...

Summary from the GEO: "Transcript analysis of RNA from murine proB cells to determine B cell repertoire and variable gene usage. RNA proB cells (B220+Cd43+IgM-CD25-) sorted from control and Setd2 knockout mice was analyzed for expression and use in the TRUST B/T cell repertoire algorithm."

Summary from the GEO: "Genetic and epigenetic intra-tumoral heterogeneity cooperate to shape the evolutionary course of cancer. In addition to genetic mutations, chronic lymphocytic leukemia (CLL) undergoes diversification through stochastic DNA methylation changes – epimutations. To measure the epimutation rate at single-cell resolution, we applied multiplexed reduced representation bisulfite sequencing...

Summary from the GEO: "Linker histone H1 proteins bind to nucleosomes and cause chromatin compaction, although little is known about their biological functions. Histone H1 (HIST1H1B-E) mutations are highly recurrent in B-cell lymphomas and are present in solid tumors, but their cancer relevance and mechanism are unknown. Here we report that lymphoma-associated H1 alleles are genetic driver mutations...

Summary from the GEO: "Changes in DNA methylation are required for the formation of germinal centers (GC), but the mechanisms of such changes are poorly understood. Activation-induced cytidine deaminase (AID) has been recently implicated recently in DNA demethylation through its deaminase activity coupled with DNA repair. We investigated the epigenetic function of AID in vivo in germinal center B...

Summary from GEO: "Following infection or vaccination, activated B cells at extrafollicular sites or within germinal centers (GCs) undergo vigorous clonal proliferation. Proliferating lymphocytes have been shown to undertake lactate dehydrogenase A (LDHA)-dependent aerobic glycolysis; however, the specific role of this metabolic pathway in a B cell transitioning from a naïve to a highly proliferative,...

Summary from GEO: "Following infection or vaccination, activated B cells at extrafollicular sites or within germinal centers (GCs) undergo vigorous clonal proliferation. Proliferating lymphocytes have been shown to undertake lactate dehydrogenase A (LDHA)-dependent aerobic glycolysis; however, the specific role of this metabolic pathway in a B cell transitioning from a naïve to a highly proliferative,...

Summary from GEO: "Following infection or vaccination, activated B cells at extrafollicular sites or within germinal centers (GCs) undergo vigorous clonal proliferation. Proliferating lymphocytes have been shown to undertake lactate dehydrogenase A (LDHA)-dependent aerobic glycolysis; however, the specific role of this metabolic pathway in a B cell transitioning from a naïve to a highly proliferative,...

Summary from GEO: "The goal of this study is to compare the transcriptomes of memory and naïve B cells, and provide a resource for single cell analysis of murine B cells during influenza infection." Overall design from GEO: "Standard droplet-based single cell RNA sequencing of naïve and memory B cells done via 10X and analyzed with Seurat."