Intrinsic Coupling of Lagging Strand Synthesis to Chromatin Assembly

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UID: 11222

Author(s): Smith, Duncan J.*, McGuffee, S., Whitehouse, Iestyn* * MSK affiliated

Description: Summary from GEO:

"We show that ligation-competent Okazaki fragments in Saccharomyces cerevisiae are sized according to the chromatin repeat. Using deep sequencing, we demonstrate that ligation junctions preferentially occur around nucleosome midpoints rather than in internucleosomal linker regions. Disrupting chromatin assembly or lagging strand polymerase processivity impacts both the size and the distribution of Okazaki fragments, suggesting a role for nascent chromatin, assembled immediately after the passage of the replication fork, in the termination of lagging strand synthesis. Our studies represent the first high-resolution analysis of eukaryotic Okazaki fragments in vivo, and establish a mechanistic link between the fundamental processes of DNA replication and chromatin assembly."

Overall design from GEO:

"4 samples: replicate samples of wild-type and pol32 knockout"

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE33786

Access via BioProject

Accession #: PRJNA148051

Access via SRA

Accession #: SRP009379
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, BioProject, and SRA databases provides open access to these files.
Associated Publications: Smith D, Whitehouse I. Intrinsic coupling of lagging-strand synthesis to chromatin assembly. Nature. 2012; 483(7390):434-438.
Equipment Used: Illumina HiSeq 2000
High throughput gene sequencing system
Dataset Format(s): TAR, BED
Dataset Size: 533.0 MB

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