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The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation

UID: 11355

Author(s): Batki, Julia, Schnabl, Jakob, Wang, Juncheng*, Handler, Dominik, Andreev, Veselin I., Stieger, Christian E., Novatchkova, Maria, Lampersberger, Lisa, Kauneckaite, Kotryna, Xie, Wei, Mechtler, Karl, Patel, Dinshaw J.*, Brennecke, Julius Fabian * MSK affiliated

Description
Summary from GEO:

"The PIWI-interacting RNA (piRNA) pathway protects animal genome integrity in part through establishing repressive heterochromatin at transposon loci. Silencing requires piRNA- guided targeting of nuclear PIWI proteins to nascent transposon transcripts, yet the subsequent molecular events are not understood. Here, we identify SFiNX (Silencing Factor interacting Nuclear eXport variant), an interdependent protein complex required for Piwi-mediated co-transcriptional silencing in Drosophila. SFiNX consists of Nxf2-Nxt1, a gonad- specific variant of the heterodimeric mRNA export receptor Nxf1-Nxt1, and the Piwi-associated protein Panoramix. SFiNX mutant flies are sterile and exhibit transposon de-repression because piRNA-loaded Piwi is unable to establish heterochromatin. Within SFiNX, Panoramix recruits the cellular heterochromatin machinery, while Nxf2 binds the nascent target RNA and licenses co-transcriptional silencing. Our results reveal an unexpected, RNA-export independent role for Nxf2 in nuclear small RNA biology and suggest that NXF-variants, which have largely unknown function in animals, are functionally linked to the genome-transposon conflict."

Overall design from GEO:

"RNA-Seq analysis of total RNAs (with rRNA depletion or polyA selection) from ovaries and ovarian stem cells (OSCs) of Nxf2, Panx, Piwi mutants."
Subject of Study
Drosophila melanogaster
Subject(s)
Genomics
Heterochromatin
RNA Interference
RNA-Binding Proteins
RNA-Seq
Access via GEO


Accession #: GSE120617

Access via BioProject


Accession #: PRJNA493759

Access via SRA


Accession #: SRP162835

Access Restrictions
Free to All
Access Instructions
The NCBI Gene Expression Omnibus, BioProject, and SRA databases provide open access to these files.
Associated Publications
Batki J, Schnabl J, Wang J, Handler D, Andreev V, Stieger C, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel D, Brennecke J. The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nature Structural and Molecular Biology. 2019; 26(8):720-731.
Equipment Used
Illumina HiSeq 2500

High throughput sequencing system

Dataset Format(s)
TAR, TXT, BW
Dataset Size
8.5 GB
Data Catalog Record Updated
2024-09-10