Summary from GEO: "RNA-seq analysis of tumor-specific CD8 T cells encountering tumor antigens with varying TCR/pMHC affinity." Overall design from GEO: "Transgenic antigen-specific CD8 T cells were adoptively transferred into tumor-bearing mice, isolated and flow-sorted from tumor draining lymph nodes and tumors at distinct time points post transfer for subsequent RNA-seq analysis. MCA 205 tumor...

Summary from GEO: "ATACseq analysis of tumor-infiltrating CD8 T cells encountering high (F6) or low (D4) affinity tumor antigen." Overall design from GEO: "Transgenic antigen-specific CD8 T cells were adoptively transferred into tumor-bearing mice, isolated and flow-sorted from tumors 14 days post-transfer for subsequent ATAC-seq analysis. MCA 205 tumor cell lines were generated to express a...

Summary from GEO: "The failure of T-cells to control tumor growth has been associated with several functional defects that collectively lead to T-cell “exhaustion.” This phenotype results from chronic antigen stimulation within the tumor microenvironment, but how repetitive antigenic stimulation leads to T-cell exhaustion remains poorly defined. Here we show that persistent antigen stimulation induces...