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  • Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells

    Authors
    Heissel, Soren
    Tavazoie, Sohail F.
    Description

    Description from PRIDE: "Proteomic changes following arginine deprivation in colorectal cancer cells before/after starvation were quantified with TMT mass spec" Sample Processing Protocol from PRIDE: "RKO cells were plated in full media in quadruplicates. Once at 40% confluence, cells were washed with PBS and continued on either full media (condition “DF” 1-4) or low-arginine media (12.5 uM -...

    Subject
    Arginine
    Colorectal Neoplasms
    Mass spectrometry
    Proteomics
    Access Rights
    Free to All
  • Ribosome dynamics following arginine deprivation in colon cancer cells

    Authors
    Hsu, Dennis J.
    Description

    Summary from GEO: "To investigate ribosome localization and gene expression dynamics during starvation in cancer cells, we performed RNA sequencing and ribosome profiling in RKO cells under fed and starved (12.5 uM arginine) conditions." Overall design from GEO: "Cells were kept under fed (400 uM arginine) or starved (12.5 uM arginine) conditions for 24 hours. RNA sequencing and ribosome profiling...

    Subject
    Arginine
    Colonic Neoplasms
    Ribosome Profiling
    RNA-Seq
    Access Rights
    Free to All