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Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells
- Authors
- Heissel, SorenTavazoie, Sohail F.
- Description
Description from PRIDE: "Proteomic changes following arginine deprivation in colorectal cancer cells before/after starvation were quantified with TMT mass spec" Sample Processing Protocol from PRIDE: "RKO cells were plated in full media in quadruplicates. Once at 40% confluence, cells were washed with PBS and continued on either full media (condition “DF” 1-4) or low-arginine media (12.5 uM -...
- Subject
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ArginineColorectal NeoplasmsMass spectrometryProteomics
- Access Rights
- Free to All
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Ribosome dynamics following arginine deprivation in colon cancer cells
- Authors
- Hsu, Dennis J.
- Description
Summary from GEO: "To investigate ribosome localization and gene expression dynamics during starvation in cancer cells, we performed RNA sequencing and ribosome profiling in RKO cells under fed and starved (12.5 uM arginine) conditions." Overall design from GEO: "Cells were kept under fed (400 uM arginine) or starved (12.5 uM arginine) conditions for 24 hours. RNA sequencing and ribosome profiling...
- Subject
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ArginineColonic NeoplasmsRibosome ProfilingRNA-Seq
- Access Rights
- Free to All