scRNA-seq to study features of CD4conv and Treg cells in spleen and bone marrow of mice

Authors

Elias, Schlomo

Schizas, Michail

Rudensky, Alexander Y.

Description

Summary from GEO: "The aim of the study was to compare gene expression between Treg cells in the bone marrow and the spleen." Overall design from GEO: "2 male Foxp3-DTR-GFP were used for the study. Single cells suspension from BM and spleen were prepared, then enriched for CD4 by magneic beads and sorted by gating on CD4+TCRb+. GFP was used to differentiate between Tregs and CD4conv. CD4conv...

Subject

Bone Marrow

CD4-Positive T-Lymphocytes

Sequence Analysis, RNA

Spleen

T-Lymphocytes, Regulatory

Access Rights

Free to All