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Results Found: 7
  • Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [2D culture]

    Authors
    Campbell, Nathaniel
    Xavier, Joao B.
    White, Richard Mark
    Description

    Summary from GEO: "We report the gene expression differences between the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV." Overall design from GEO: "RNA-seq of ZMEL1-PRO and ZMEL1-INV cells grown in standard adherent (2D) culture."

    Subject
    Cell Culture Techniques
    Cell Line, Tumor
    Melanoma
    Neoplasm Metastasis
    RNA-Seq
    Access Rights
    Free to All
  • HEK293T cell translatome analysis upon 6-MP treatment. Related to Supplementary Table 6 from An et al (2020)

    Authors
    Ordureau, Alban Simon Georges
    Harper, Wade J.
    Description

    HEK293T cells were treated with 6-MP and harvested at various time point (6, 9 and 18h). Translatome was measure by Azidohomoalanine (AHA) incorporation, Biotin-click, and streptavidin enrichment.

    Subject
    Biotin
    Cell Culture Techniques
    HEK293 Cells
    Mass spectrometry
    Access Rights
    Free to All
  • KO_Vehicle_H3K27me3

    Description

    Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....

    Subject
    Cell Culture Techniques
    Chromatin Immunoprecipitation Sequencing
    Gene Knockout Techniques
    Histones
    Access Rights
    Free to All
  • WT_Vehicle_H3K27me3

    Description

    Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....

    Subject
    Cell Culture Techniques
    Chromatin Immunoprecipitation Sequencing
    DNA Fragmentation
    Histones
    Access Rights
    Free to All
  • KO_Vehicle_H3K27ac

    Description

    Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....

    Subject
    Cell Culture Techniques
    Chromatin Immunoprecipitation Sequencing
    Gene Knockout Techniques
    Histones
    Access Rights
    Free to All
  • WT_Vehicle_H3K27ac

    Description

    Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....

    Subject
    Cell Culture Techniques
    Chromatin Immunoprecipitation Sequencing
    DNA Fragmentation
    Histones
    Access Rights
    Free to All
  • Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through a NGFR-dependent mechanism (EV-track samples)

    Authors
    García-Silva, Susana
    Benito-Martín, Alberto
    Nogués, Laura
    Hernández-Barranco, Alberto
    33 more author(s)...
    Description

    9 cell culture supernatant samples from "Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through a NGFR-dependent mechanism." Each sample reports a combination of EV-enriched proteins, non EV-enriched proteins, density gradient, ultracentrifugation specifics, antibody specifics, and/or lysate preparation.

    Subject
    Cell Culture Techniques
    Extracellular Vesicles
    Lymph Nodes
    Melanoma
    Neoplasm Metastasis
    Ultracentrifugation/methods
    Access Rights
    Free to All