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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [2D culture]
- Authors
- Campbell, NathanielXavier, Joao B.White, Richard Mark
- Description
Summary from GEO: "We report the gene expression differences between the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV." Overall design from GEO: "RNA-seq of ZMEL1-PRO and ZMEL1-INV cells grown in standard adherent (2D) culture."
- Subject
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Cell Culture TechniquesCell Line, TumorMelanomaNeoplasm MetastasisRNA-Seq
- Access Rights
- Free to All
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HEK293T cell translatome analysis upon 6-MP treatment. Related to Supplementary Table 6 from An et al (2020)
- Authors
- Ordureau, Alban Simon GeorgesHarper, Wade J.
- Description
HEK293T cells were treated with 6-MP and harvested at various time point (6, 9 and 18h). Translatome was measure by Azidohomoalanine (AHA) incorporation, Biotin-click, and streptavidin enrichment.
- Subject
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BiotinCell Culture TechniquesHEK293 CellsMass spectrometry
- Access Rights
- Free to All
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KO_Vehicle_H3K27me3
- Description
Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....
- Subject
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Cell Culture TechniquesChromatin Immunoprecipitation SequencingGene Knockout TechniquesHistones
- Access Rights
- Free to All
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KO_Vehicle_H3K27ac
- Description
Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....
- Subject
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Cell Culture TechniquesChromatin Immunoprecipitation SequencingGene Knockout TechniquesHistones
- Access Rights
- Free to All
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WT_Vehicle_H3K27me3
- Description
Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....
- Subject
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Cell Culture TechniquesChromatin Immunoprecipitation SequencingDNA FragmentationHistones
- Access Rights
- Free to All
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WT_Vehicle_H3K27ac
- Description
Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....
- Subject
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Cell Culture TechniquesChromatin Immunoprecipitation SequencingDNA FragmentationHistones
- Access Rights
- Free to All
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Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through a NGFR-dependent mechanism (EV-track samples)
- Authors
- García-Silva, SusanaBenito-Martín, AlbertoNogués, LauraHernández-Barranco, Alberto33 more author(s)...
- Description
9 cell culture supernatant samples from "Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through a NGFR-dependent mechanism." Each sample reports a combination of EV-enriched proteins, non EV-enriched proteins, density gradient, ultracentrifugation specifics, antibody specifics, and/or lysate preparation.
- Subject
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Cell Culture TechniquesExtracellular VesiclesLymph NodesMelanomaNeoplasm MetastasisUltracentrifugation/methods
- Access Rights
- Free to All