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Single-cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment - 5' RNA sequencing and TCR sequencing
- Authors
- Azizi, ElhamCarr, Ambrose JamesPlitas, GeorgeCornish, Andrew Erickson15 more author(s)...
- Description
Single-cell RNA sequencing was performed on three patients using the 10x genomics TCR profiling kits. For each patient, populations of T-cells were assayed for both TCR sequence and trancriptome-wide RNA-sequence. Two donors have a replicate experiment. From the GEO summary: "Knowledge of immune cell phenotypes in the tumor microenvironment is essential for understanding mechanisms of cancer progression...
- Subject
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Breast NeoplasmsGene Expression ProfilingGene Expression Regulation, NeoplasticImmunotherapyLymphocytes, Tumor-InfiltratingReceptors, Antigen, T-CellTranscriptomeTumor Microenvironment/immunology
- Access Rights
- Free to All
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Single-cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment 3' RNA Sequencing
- Authors
- Azizi, ElhamCarr, Ambrose JamesPlitas, GeorgeCornish, Andrew Erickson15 more author(s)...
- Description
Single-cell RNA sequencing was performed on eight donors using the InDrop v2 protocol. For each donor populations of CD45+ immune cells were assayed for trancriptome-wide RNA-sequence. At least one replicate was taken for each donor. From the GEO summary: "Knowledge of immune cell phenotypes in the tumor microenvironment is essential for understanding mechanisms of cancer progression and immunotherapy...
- Subject
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Breast NeoplasmsGene Expression ProfilingGene Expression Regulation, NeoplasticImmunotherapyLymphocytes, Tumor-InfiltratingReceptors, Antigen, T-CellTranscriptomeTumor Microenvironment/immunology
- Access Rights
- Free to All
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Flow Cytometry Datasets Associated with Single-cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment research
- Authors
- Cornish, Andrew Erickson
- Description
The purpose of the dataset from the Flow Repository: "To determine the degree to which populations of intratumoral T cells, especially but not limited to T regs, initially found in single cell RNA sequencing data may be reproduced in mass cytometry data." From the description on Flow Repository: The data included for each of the 3 patients (BC12, BC13, and BC14, respectively) is presented in nine...
- Subject
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Breast NeoplasmsGene Expression ProfilingGene Expression Regulation, NeoplasticImmunotherapyLeukocytes, MononuclearLymphocytes, Tumor-InfiltratingTumor Microenvironment/immunology
- Timeframe
- 2018
- Access Rights
- Free to All