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  • A lymphatic-stem cell interactome regulates intestinal stem cell activity

    Authors
    Niec, Rachel E.
    Chu, Tin Yi
    Gur-Cohen, Shiri
    Schernthanner, Marina
    2 more author(s)...
    Description

    Summary from GEO: "Single-cell RNA-sequencing of murine small intestinal organoid cultured with and without lymphatic endothelial cells: Purpose: To identify differences in cell composition in murine organoids upon coculture with lymphatic endothelial cells Results: We recovered 818 cells with a median of 37750 UMIs per cell for the organoid alone and 1233 cells with a median of 79354 UMIs per cells...

    Subject
    Endothelial Cells
    Intestinal Mucosa
    RNA-Seq
    Single-Cell Analysis
    Stem Cells
    Transcriptome
    Access Rights
    Free to All
  • Bulk RNA-Sequencing of mouse large intestinal lymphatics (capillaries and collectors)

    Authors
    Niec, Rachel E.
    Chu, Tin Yi
    Gur-Cohen, Shiri
    Schernthanner, Marina
    2 more author(s)...
    Description

    Summary from GEO: "Purpose: Profile global gene expression of intestinal lymphatics to compare with published datasets Methods: Intestinal tissues were digested, lymphatic endothelial cells were sorted using lymphatic markers including CD31, Podoplanin, Lyve1. Total RNA was isolated from sorted cells using the Direct-Zol RNA MiniPrep kit (Zymo). Samples were submitted for bulk RNA-Sequencing. Conclusions:...

    Subject
    Endothelial Cells
    Gene Expression Profiling
    Intestinal Mucosa
    RNA-Seq
    Access Rights
    Free to All