CRISPR enhancer scan to detect critical elements for the regulation of EVI1 in a translocated enhancer

Authors

Delwel, Ruud

Description

Description from EMBL-EBI: "To uncover, in an unbiased fashion, which elements of the 18 kb translocated region control EVI1 transcription, we devised a CRISPR/Cas9-based enhancer scanning approach. We considered all possible sgRNA target sites containing a canonical Cas9 PAM site (NGG) on both strands of the minimal 18 kb translocated region. Deep-sequencing libraries were generated by PCR amplification...

Subject

CRISPR-Cas Systems

High-Throughput Nucleotide Sequencing

Leukemia

MDS1 and EVI1 Complex Locus Protein

Polymerase Chain Reaction

Access Rights

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