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  • Replication-coupled nucleosome assembly and positioning by ATP-dependent chromatin remodeling enzymes

    Authors
    Yadav, Tejas
    Whitehouse, Iestyn
    Description

    Summary from GEO: "We have previously shown that Okazaki fragments in Saccharomyces cerevisiae are sized according to the chromatin repeat. Here we demonstrate that nucleosome positioning is rapidly established on newly synthesized DNA. Using deep sequencing, we demonstrate that ATP-dependent chromatin remodeling enzymes, Isw1 and Chd1, collaborate with histone chaperones, such as CAF-1 and Rtt106,...

    Subject
    Chromatin
    DNA Ligase ATP
    DNA Replication
    High-Throughput Nucleotide Sequencing
    Nucleosomes
    Access Rights
    Free to All
  • Nucleosome Positioning Dynamics Through the Yeast Metabolic Cycle

    Authors
    Nocetti, Nicolas
    Whitehouse, Iestyn
    Description

    Summary from GEO: "We present Micrococcal Nuclease digestion maps of S. cerevisiae through the progression of the Yeast Metabolic Cycle. We demonstrate that nucleosome positions at many promoters are dynamic, and remodeling events at promoters have significant consequences with respect to gene expression." Overall design from GEO: "Examination of nucleosome positions and transcriptional output...

    Subject
    Gene Expression
    Micrococcal Nuclease
    Nucleosomes
    Transcription, Genetic
    Yeasts/metabolism
    Access Rights
    Free to All