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Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis (CHX experiments)
- Authors
- Garg, AngadShuman, Stewart H.Schwer, Beate
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of fission yeast cells (Schizosaccharomyces pombe) at various times after phosphate starvation (2, 4, and 8 HR) either with 100 µg/ml cycloheximide or without drug contrasting to the cells grown in phosphate replete conditions (0HR) in the absence of the drug." Overall design from GEO: "6 test samples (cycloheximide or no drug...
- Subject
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CycloheximidePhosphates/metabolismRNA-SeqSchizosaccharomycesSchizosaccharomyces pombe Proteins
- Access Rights
- Free to All
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Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis
- Authors
- Garg, AngadShuman, Stewart H.
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of fission yeast (Schizosaccharomyces pombe) strains prior to phosphate starvation (0 HR) and at various times after phosphate starvation (4, 8, 12, 24, 36, and 48 HR) contrasting to the WT fission yeast S. pombe cells grown in phosphate replete conditions (WT 0HR)." Overall design from GEO: "13 test samples and 1 control sample...
- Subject
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Phosphates/metabolismRNA-SeqSchizosaccharomycesSchizosaccharomyces pombe Proteins
- Access Rights
- Free to All
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Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis (mass spectrometry proteomics data)
- Authors
- Garg, AngadShuman, Stewart H.
- Description
Inorganic phosphate is an essential nutrient acquired by cells from their environment. Here we characterize the adaptative responses of fission yeast to chronic phosphate starvation, during which cells enter a state of quiescence, initially fully reversible upon replenishing phosphate after 2 days but resulting in gradual loss of viability during 4 weeks of starvation. Time-resolved analyses of changes...
- Subject
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Liquid Chromatography-Mass SpectrometryMaf Transcription FactorsPhosphates/metabolismProtease InhibitorsProteomeRNA, Transfer
- Access Rights
- Free to All