Search Tips
of 1 Next >
Results Found: 1
  • CRISPR enhancer scan to detect critical elements for the regulation of EVI1 in a translocated enhancer

    Authors
    Delwel, Ruud
    Description

    Description from EMBL-EBI: "To uncover, in an unbiased fashion, which elements of the 18 kb translocated region control EVI1 transcription, we devised a CRISPR/Cas9-based enhancer scanning approach. We considered all possible sgRNA target sites containing a canonical Cas9 PAM site (NGG) on both strands of the minimal 18 kb translocated region. Deep-sequencing libraries were generated by PCR amplification...

    Subject
    CRISPR-Cas Systems
    High-Throughput Nucleotide Sequencing
    Leukemia
    MDS1 and EVI1 Complex Locus Protein
    Polymerase Chain Reaction
    Access Rights
    Free to All