Summary from the GEO: "Programmed DNA double-strand breaks (DSBs) initiate meiotic recombination and their subsequent repair culminates in crossover (CO) formation. COs result from the asymmetric cleavage of double-Holliday junction (dHJ) intermediates, that requires the MutLγ complex together with a non-catalytic function of Exo1, an activity essential for fertility but at risk of generating unwanted...

Summary from the GEO: "Programmed DNA double-strand breaks (DSBs) initiate meiotic recombination and their subsequent repair culminates in crossover (CO) formation. COs result from the asymmetric cleavage of double-Holliday junction (dHJ) intermediates, that requires the MutLγ endonuclease and a non-catalytic function of Exo1, an activity essential for fertility but at risk of generating unwanted...

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes x-ray diffraction data used for modeling the crystal structure of a nanobody-stabilized active state of the kappa-opioid receptor. The main summary display for this entry includes information on the experimental data, validation, macromolecules, small molecules, version history and funding information....

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes x-ray diffraction  data identifying the crystal structure of Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal II sorting motif. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included...

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes x-ray diffraction  data identifying the crystal structure of Sec23a/Sec24a/Sec22 complexed with Emp24 sorting motif. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included in the deposit, and version...

Inflammasomes are multiprotein complexes formed in response to pathogens. NLRP1 and CARD8 are related proteins that form inflammasomes, but the pathogen-associated signal(s) and the molecular mechanisms controlling their activation have not been established. Inhibitors of the serine dipeptidyl peptidases DPP8 and DPP9 (DPP8/9) were recently discovered to activate both NLRP1 and CARD8. Interestingly,...

Mutant, truncated and wild-type human calreticul was co-purified with binding proteins

Affinity enrichment of ligand to mutant and wild-type PP2A protein

To find cellular protein binding partners of the NLRP1 inflammasome, FLAG-tagged full length NLRP1 (or a GFP-FLAG protein control) was ectopically expressed in HEK 293T cells and affinity purified from cell lysates (Note: the NLRP1-FLAG-containing lysate samples were affinity purified in the presence or absence of excess proline) before proteins were reduced, alkylated, and trypsinized before TMT...