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Results Found: 9
  • High throughput joint profiling of chromatin accessibility and protein levels in single cells [Tcell KO]

    Authors
    Mimitou, Eleni P.
    Lareau, Caleb A.
    Chen, Kelvin Y.
    Ludwig, Leif S.
    1 more author(s)...
    Description

    Summary from GEO: "Recent technological advances have enabled massively parallel single-cell Assay for Transposase Accessible Chromatin by sequencing (scATAC-seq) to simultaneously profile the epigenomic landscape in thousands of individual cells. scATAC-seq methods sample genomic DNA accessible to transposases, but have not previously been combined with measurement of protein levels. Here, we present...

    Subject
    Chromatin Immunoprecipitation Sequencing
    Gene Expression Profiling
    Gene Knockout Techniques
    Hematopoiesis
    RNA, Guide, CRISPR-Cas Systems
    T-Lymphocytes
    Access Rights
    Free to All
  • Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation

    Authors
    Huang, Hsin-Che
    Orth-He, Elizabeth L.
    Bachovchin, Daniel
    Description

    Summary from GEO: "We report the application of RNA-seq for profiling gene transcription upon treatment of methyl bestatin (MeBs, aminopeptidase inhibitor) or brefeldin A (BFA, ER-to-Golgi transport inhibitor), compared to DMSO control. We found MeBs and BFA similarly upregulated genes pertinent to proteotoxic stress." Overall design from GEO: "Examination of gene transcription levels upon treatment...

    Subject
    Gene Knockout Techniques
    Inflammasomes
    Protein Folding
    Proteotoxic Stress
    Access Rights
    Free to All
  • CD32A Immunoprecipitation in K562 Cell Membrane and Cytoplasm Fractions

    Authors
    Li, Zhuoning
    Abdel-Wahab, Omar Ibrahim
    Description

    Description from PRIDE: "Here we interrogate CD32A protein interactions in the cytoplasm and membrane fractions isolated from the human AML cell lines K562 wild-type and knockout for CD32A. Cells were fractionated prior to incubation with CD32A antibody and subsequent samples isolated using protein agarose beads."

    Subject
    Cell Line, Tumor
    Cytoplasm
    Gene Knockout Techniques
    Leukemia, Myeloid, Acute
    Access Rights
    Free to All
  • CUT&RUN Supplementary Data from Smarca4 Inactivation Promotes Lineage-Specific Transformation and Early Metastatic Features in the Lung

    Authors
    Concepcion, Carla P.
    Ma, Sai
    LaFave, Lindsay M.
    Bhutkar, Arjun
    19 more author(s)...
    Description

    Description from Figshare: "Processed data for CUT&RUN of isogenic SMARCA4-WT and -KO cell lines"

    Subject
    Adenocarcinoma of Lung
    Cell Lineage
    Chromatin Immunoprecipitation Sequencing
    Gene Knockout Techniques
    Lung Neoplasms
    Neoplasm Metastasis
    Transcription Factors
    Access Rights
    Free to All
  • Supplemental Table S2 from Histone Acetyltransferase Activity of MOF Is Required for MLL-AF9 Leukemogenesis

    Authors
    Valerio, Daria G.
    Xu, Haiming
    Chen, Chun-Wei
    Hoshii, Takayuki
    11 more author(s)...
    Description

    Description from Figshare: "RNA-seq MOF KO vs MOF WT"

    Subject
    Gene Knockout Techniques
    Histone Acetyltransferases
    Leukemia
    RNA-Seq
    Access Rights
    Free to All
  • Intrinsic Coupling of Lagging Strand Synthesis to Chromatin Assembly

    Authors
    Smith, Duncan J.
    McGuffee, S.
    Whitehouse, Iestyn
    Description

    Summary from GEO: "We show that ligation-competent Okazaki fragments in Saccharomyces cerevisiae are sized according to the chromatin repeat. Using deep sequencing, we demonstrate that ligation junctions preferentially occur around nucleosome midpoints rather than in internucleosomal linker regions. Disrupting chromatin assembly or lagging strand polymerase processivity impacts both the size and...

    Subject
    Chromatin
    Chromatin Assembly and Disassembly
    DNA Ligases
    DNA Replication
    Gene Knockout Techniques
    Nucleotides
    Access Rights
    Free to All
  • KO_Vehicle_H3K27me3

    Description

    Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....

    Subject
    Cell Culture Techniques
    Chromatin Immunoprecipitation Sequencing
    Gene Knockout Techniques
    Histones
    Access Rights
    Free to All
  • KO_Vehicle_H3K27ac

    Description

    Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....

    Subject
    Cell Culture Techniques
    Chromatin Immunoprecipitation Sequencing
    Gene Knockout Techniques
    Histones
    Access Rights
    Free to All
  • Transcriptional response of mammalian cells lacking FLVCR1 to choline depleted or replete media conditions

    Authors
    Birsoy, Kivanç
    Kenny, Timothy C.
    Description

    Summary from GEO: "Integrative genetic analysis identified FLVCR1 as a choline transporter in mammalian cells. We profiled the transcriptional response of HeLa cells with and without FLVCR1 to choline depleted or replete conditions." Overall design from GEO: "Comparative gene expression profiling analysis of RNA-seq data from FLVCR1 knockout HeLa cells complemented with FLVCR1 cDNA or vector...

    Subject
    Choline
    Gene Expression Profiling
    Gene Knockout Techniques
    Membrane Transport Proteins
    RNA-Seq
    Access Rights
    Free to All