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High throughput joint profiling of chromatin accessibility and protein levels in single cells [Tcell KO]
- Authors
- Mimitou, Eleni P.Lareau, Caleb A.Chen, Kelvin Y.Ludwig, Leif S.1 more author(s)...
- Description
Summary from GEO: "Recent technological advances have enabled massively parallel single-cell Assay for Transposase Accessible Chromatin by sequencing (scATAC-seq) to simultaneously profile the epigenomic landscape in thousands of individual cells. scATAC-seq methods sample genomic DNA accessible to transposases, but have not previously been combined with measurement of protein levels. Here, we present...
- Subject
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Chromatin Immunoprecipitation SequencingGene Expression ProfilingGene Knockout TechniquesHematopoiesisRNA, Guide, CRISPR-Cas SystemsT-Lymphocytes
- Access Rights
- Free to All
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Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation
- Authors
- Huang, Hsin-CheOrth-He, Elizabeth L.Bachovchin, Daniel
- Description
Summary from GEO: "We report the application of RNA-seq for profiling gene transcription upon treatment of methyl bestatin (MeBs, aminopeptidase inhibitor) or brefeldin A (BFA, ER-to-Golgi transport inhibitor), compared to DMSO control. We found MeBs and BFA similarly upregulated genes pertinent to proteotoxic stress." Overall design from GEO: "Examination of gene transcription levels upon treatment...
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Gene Knockout TechniquesInflammasomesProtein FoldingProteotoxic Stress
- Access Rights
- Free to All
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CD32A Immunoprecipitation in K562 Cell Membrane and Cytoplasm Fractions
- Authors
- Li, ZhuoningAbdel-Wahab, Omar Ibrahim
- Description
Description from PRIDE: "Here we interrogate CD32A protein interactions in the cytoplasm and membrane fractions isolated from the human AML cell lines K562 wild-type and knockout for CD32A. Cells were fractionated prior to incubation with CD32A antibody and subsequent samples isolated using protein agarose beads."
- Subject
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Cell Line, TumorCytoplasmGene Knockout TechniquesLeukemia, Myeloid, Acute
- Access Rights
- Free to All
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CUT&RUN Supplementary Data from Smarca4 Inactivation Promotes Lineage-Specific Transformation and Early Metastatic Features in the Lung
- Authors
- Concepcion, Carla P.Ma, SaiLaFave, Lindsay M.Bhutkar, Arjun19 more author(s)...
- Description
Description from Figshare: "Processed data for CUT&RUN of isogenic SMARCA4-WT and -KO cell lines"
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Adenocarcinoma of LungCell LineageChromatin Immunoprecipitation SequencingGene Knockout TechniquesLung NeoplasmsNeoplasm MetastasisTranscription Factors
- Access Rights
- Free to All
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Supplemental Table S2 from Histone Acetyltransferase Activity of MOF Is Required for MLL-AF9 Leukemogenesis
- Authors
- Valerio, Daria G.Xu, HaimingChen, Chun-WeiHoshii, Takayuki11 more author(s)...
- Description
Description from Figshare: "RNA-seq MOF KO vs MOF WT"
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Gene Knockout TechniquesHistone AcetyltransferasesLeukemiaRNA-Seq
- Access Rights
- Free to All
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Intrinsic Coupling of Lagging Strand Synthesis to Chromatin Assembly
- Authors
- Smith, Duncan J.McGuffee, S.Whitehouse, Iestyn
- Description
Summary from GEO: "We show that ligation-competent Okazaki fragments in Saccharomyces cerevisiae are sized according to the chromatin repeat. Using deep sequencing, we demonstrate that ligation junctions preferentially occur around nucleosome midpoints rather than in internucleosomal linker regions. Disrupting chromatin assembly or lagging strand polymerase processivity impacts both the size and...
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ChromatinChromatin Assembly and DisassemblyDNA LigasesDNA ReplicationGene Knockout TechniquesNucleotides
- Access Rights
- Free to All
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KO_Vehicle_H3K27me3
- Description
Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....
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Cell Culture TechniquesChromatin Immunoprecipitation SequencingGene Knockout TechniquesHistones
- Access Rights
- Free to All
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KO_Vehicle_H3K27ac
- Description
Extraction protocol from GEO: "Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest. Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification....
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Cell Culture TechniquesChromatin Immunoprecipitation SequencingGene Knockout TechniquesHistones
- Access Rights
- Free to All
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Transcriptional response of mammalian cells lacking FLVCR1 to choline depleted or replete media conditions
- Authors
- Birsoy, KivançKenny, Timothy C.
- Description
Summary from GEO: "Integrative genetic analysis identified FLVCR1 as a choline transporter in mammalian cells. We profiled the transcriptional response of HeLa cells with and without FLVCR1 to choline depleted or replete conditions." Overall design from GEO: "Comparative gene expression profiling analysis of RNA-seq data from FLVCR1 knockout HeLa cells complemented with FLVCR1 cDNA or vector...
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CholineGene Expression ProfilingGene Knockout TechniquesMembrane Transport ProteinsRNA-Seq
- Access Rights
- Free to All