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Results Found: 20
  • MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells

    Authors
    Wei, Qiaozhi
    Frenette, Paul S.
    Description

    Summary from the GEO: "Haematopoietic stem cells (HSCs) tightly regulate their quiescence, proliferation, and differentiation to generate blood cells during the entire lifetime. The mechanisms by which these critical activities are balanced are still unclear. Here, we report that Macrophage-Erythroblast Attacher (MAEA, also known as EMP), a receptor thus far only identified in erythroblastic island1,...

    Subject
    Cell Adhesion Molecules
    Cytoskeletal Proteins
    Gene Expression Profiling
    Hematopoiesis
    Hematopoietic Stem Cells
    Leukemia, Myeloid, Acute
    Lymphokines
    Ubiquitin-Protein Ligases
    Access Rights
    Free to All
  • Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis [ATAC-Seq]

    Authors
    Ott, Christopher J.
    Viny, Aaron David
    Description

    Summary of the GEO: "Cohesin complex members have recently been identified as putative tumor suppressors in hematologic and epithelial malignancies. The cohesin complex guides chromosome segregation, however cohesin-mutant leukemias do not show genomic instability. We hypothesized reduced cohesin function alters chromatin structure and disrupts cis-regulatory architecture of hematopoietic progenitors....

    Subject
    fms-Like Tyrosine Kinase 3
    Hematopoiesis
    Leukemia, Myeloid, Acute
    Proto-Oncogene Proteins c-kit
    Access Rights
    Free to All
  • Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis [RNA-Seq]

    Authors
    Ott, Christopher J.
    Viny, Aaron David
    Description

    Summary from the GEO: "Cohesin complex members have recently been identified as putative tumor suppressors in hematologic and epithelial malignancies. The cohesin complex guides chromosome segregation, however cohesin-mutant leukemias do not show genomic instability. We hypothesized reduced cohesin function alters chromatin structure and disrupts cis-regulatory architecture of hematopoietic progenitors....

    Subject
    fms-Like Tyrosine Kinase 3
    Hematopoiesis
    Leukemia, Myeloid, Acute
    Proto-Oncogene Proteins c-kit
    Access Rights
    Free to All
  • Stag1 and Stag2 regulate cell fate decisions in hematopoiesis through non-redundant topological control [II]

    Authors
    Viny, Aaron David
    Lavallée, Vincent-Phillipe
    Levine, Ross L.
    Description

    Summary from GEO: "Transcriptional regulators, including the cohesin complex member STAG2, are recurrently mutated in cancer. The role of STAG2 in gene regulation, hematopoiesis, and tumor suppression remains unresolved. We show Stag2 deletion in hematopoietic stem/progenitor cells (HSPC) results in altered hematopoietic function, increased self-renewal, and impaired differentiation. ChIP-sequencing...

    Subject
    Binding sites
    Gene Expression Regulation
    Hematopoiesis
    Hematopoietic Stem Cells
    RNA-Seq
    Access Rights
    Free to All
  • Stag1 and Stag2 regulate cell fate decisions in hematopoiesis through non-redundant topological control [I]

    Authors
    Viny, Aaron David
    Levine, Ross L.
    Koche, Richard Patrick
    Description

    Summary from GEO: "Transcriptional regulators, including the cohesin complex member STAG2, are recurrently mutated in cancer. The role of STAG2 in gene regulation, hematopoiesis, and tumor suppression remains unresolved. We show Stag2 deletion in hematopoietic stem/progenitor cells (HSPC) results in altered hematopoietic function, increased self-renewal, and impaired differentiation. ChIP-sequencing...

    Subject
    Binding sites
    Gene Expression Regulation
    Hematopoiesis
    Hematopoietic Stem Cells
    RNA-Seq
    Access Rights
    Free to All
  • Stag2 regulates hematopoietic differentiation and self-renewal through alterations in gene expression and topological control

    Authors
    Liu, Yu
    Dekker, Job
    Description

    Summary from GEO: "We performed HiC analyzed on wildtype and Stag2 knock-out hematopoietic progeitor cells and integrated with Stag2 ChIP-seq and RNAseq data to identy genes whose expression and tological structures are regulated by Stag2 during hematopoietic differentiation and self-renewal." Overall design from GEO: "Comparing HiC profiles of two wildtype hematopoietic progenitor cells with...

    Subject
    Binding sites
    Chromatin Immunoprecipitation Sequencing
    Gene Expression Regulation
    Hematopoiesis
    Hematopoietic Stem Cells
    RNA-Seq
    Access Rights
    Free to All
  • High throughput joint profiling of chromatin accessibility and protein levels in single cells [PBMC stimulation]

    Authors
    Mimitou, Eleni P.
    Lareau, Caleb A.
    Chen, Kelvin Y.
    Ludwig, Leif S.
    1 more author(s)...
    Description

    Summary from GEO: "Recent technological advances have enabled massively parallel single-cell Assay for Transposase Accessible Chromatin by sequencing (scATAC-seq) to simultaneously profile the epigenomic landscape in thousands of individual cells. scATAC-seq methods sample genomic DNA accessible to transposases, but have not previously been combined with measurement of protein levels. Here, we present...

    Subject
    Cell Differentiation
    Chromatin Immunoprecipitation Sequencing
    Gene Expression Profiling
    Hematopoiesis
    Access Rights
    Free to All
  • High throughput joint profiling of chromatin accessibility and protein levels in single cells [PBMCs]

    Authors
    Mimitou, Eleni P.
    Lareau, Caleb A.
    Chen, Kelvin Y.
    Ludwig, Leif S.
    1 more author(s)...
    Description

    Summary from GEO: "Recent technological advances have enabled massively parallel single-cell Assay for Transposase Accessible Chromatin by sequencing (scATAC-seq) to simultaneously profile the epigenomic landscape in thousands of individual cells. scATAC-seq methods sample genomic DNA accessible to transposases, but have not previously been combined with measurement of protein levels. Here, we present...

    Subject
    Cell Differentiation
    Gene Expression Profiling
    Hematopoiesis
    Leukocytes, Mononuclear
    Access Rights
    Free to All
  • High throughput joint profiling of chromatin accessibility and protein levels in single cells [Tcell KO]

    Authors
    Mimitou, Eleni P.
    Lareau, Caleb A.
    Chen, Kelvin Y.
    Ludwig, Leif S.
    1 more author(s)...
    Description

    Summary from GEO: "Recent technological advances have enabled massively parallel single-cell Assay for Transposase Accessible Chromatin by sequencing (scATAC-seq) to simultaneously profile the epigenomic landscape in thousands of individual cells. scATAC-seq methods sample genomic DNA accessible to transposases, but have not previously been combined with measurement of protein levels. Here, we present...

    Subject
    Chromatin Immunoprecipitation Sequencing
    Gene Expression Profiling
    Gene Knockout Techniques
    Hematopoiesis
    RNA, Guide, CRISPR-Cas Systems
    T-Lymphocytes
    Access Rights
    Free to All
  • High throughput joint profiling of chromatin accessibility and protein levels in single cells [Tcell_Intra]

    Authors
    Mimitou, Eleni P.
    Lareau, Caleb A.
    Chen, Kelvin Y.
    Ludwig, Leif S.
    1 more author(s)...
    Description

    Summary from GEO: "Recent technological advances have enabled massively parallel single-cell Assay for Transposase Accessible Chromatin by sequencing (scATAC-seq) to simultaneously profile the epigenomic landscape in thousands of individual cells. scATAC-seq methods sample genomic DNA accessible to transposases, but have not previously been combined with measurement of protein levels. Here, we present...

    Subject
    Chromatin Immunoprecipitation Sequencing
    Gene Expression Profiling
    Hematopoiesis
    RNA, Guide, CRISPR-Cas Systems
    T-Lymphocytes
    Access Rights
    Free to All