Summary from the GEO: "RNA-sequencing on human leukemia cell lines MOLM13 (AML, MLL-AF9+) and K562 (CML-BC, BCR-ABL+) after Ro 08-2750 treatment (20 uM, 4hours). Cells were cultured at 0.5M/mL and treated with the small-molecule Ro 08-2750 for 4hours at 20 uM." The dataset is associated with the MUSASHI (MSI) family of RNA binding proteins (MSI1 and MSI2). According to the associated article, the...

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the RNA-recognition motif 1 of human MSI2. The main summary display for this entry includes an experimental data snapshot, protein source, macromolecules and small molecules included in the deposit, and version history and funding identification....

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of Csm-crRNA binary complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included in the deposit,...

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of ternary Csm-crRNA-target RNA with anti-tag sequence complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small...

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of larger Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included...

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included in...

This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Crystal structure of Csm1-Csm4 subcomplex in the type III-A CRISPR-Csm interference complex. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included in...

The SuperSeiries holds data on RNA-Sequencing in connection to HyperTRIBE in humans and mice. Each of the SubSeries details the specific use of the data and its connection to leukemia. This SuperSeries is composed of the following SubSeries: GSE132896 HyperTRIBE uncovers MSI2 increased RNA binding activity and differential regulation in leukemic stem cells [MOLM-13] GSE132947 HyperTRIBE uncovers...

Summary from the GEO: " Understanding RBPs’ molecular functions as well as their cell-type specific activity requires identification of RBPs’ direct mRNA targets. However, efforts to identify RNA targets of RNA binding proteins in stem cells have been hindered by limited cell numbers. Here we adapted the HyperTRIBE method using an RBP fused to a Drosophila RNA editing enzyme (ADAR) to allow for global...

Summary from the GEO: "Understanding RBPs’ molecular functions as well as their cell-type specific activity requires identification of RBPs’ direct mRNA targets. However, efforts to identify RNA targets of RNA binding proteins in stem cells have been hindered by limited cell numbers. Here we adapted the HyperTRIBE method using an RBP fused to a Drosophila RNA editing enzyme (ADAR) to allow for global...