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  • scRNA-seq to study features of CD4conv and Treg cells in spleen and bone marrow of mice

    Authors
    Elias, Schlomo
    Schizas, Michail
    Rudensky, Alexander Y.
    Description

    Summary from GEO: "The aim of the study was to compare gene expression between Treg cells in the bone marrow and the spleen." Overall design from GEO: "2 male Foxp3-DTR-GFP were used for the study. Single cells suspension from BM and spleen were prepared, then enriched for CD4 by magneic beads and sorted by gating on CD4+TCRb+. GFP was used to differentiate between Tregs and CD4conv. CD4conv...

    Subject
    Bone Marrow
    CD4-Positive T-Lymphocytes
    Sequence Analysis, RNA
    Spleen
    T-Lymphocytes, Regulatory
    Access Rights
    Free to All
  • Next Generation Sequencing Analysis of OX40hi Treg and OX40lo Treg cells from mouse tumors and spleens

    Authors
    Tan, Adrian
    Yang, Ning
    Deng, Liang
    Description

    Summary from GEO: "Based on RNA-seq technology, we studies gene expression profiles in Treg cells isolated from mouse tumors and spleens. We identified differential gene expressions comparing OX40hi Treg with OX40lo Treg cells from tumors and spleens. The difference of Trges in tumors and spleens were also identified." Overall design from GEO: "Foxp3-GFP mice were intradermally implanted with...

    Subject
    OX40 Ligand
    RNA-Binding Proteins
    Spleen
    T-Lymphocytes, Regulatory
    Access Rights
    Free to All