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Effect of Ro 08-2750 treatment on gene expression of human leukemia cell lines MOLM13 and K562
- Authors
- Minuesa, Gerard DinaresPassarelli, Maria C.Fairchild, Lauren E.Leslie, Christina1 more author(s)...
- Description
Summary from the GEO: "RNA-sequencing on human leukemia cell lines MOLM13 (AML, MLL-AF9+) and K562 (CML-BC, BCR-ABL+) after Ro 08-2750 treatment (20 uM, 4hours). Cells were cultured at 0.5M/mL and treated with the small-molecule Ro 08-2750 for 4hours at 20 uM." The dataset is associated with the MUSASHI (MSI) family of RNA binding proteins (MSI1 and MSI2). According to the associated article, the...
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Leukemia, Myeloid, AcuteRNA-Binding Proteins
- Access Rights
- Free to All
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RNA-recognition motif 1 of human MSI2
- Authors
- Goldgur, YehudaKharas, Michael G.Minuesa, Gerard Dinares
- Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the RNA-recognition motif 1 of human MSI2. The main summary display for this entry includes an experimental data snapshot, protein source, macromolecules and small molecules included in the deposit, and version history and funding identification....
- Subject
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Protein Structure, QuaternaryProtein Structure, SecondaryProtein Structure, TertiaryRNA-Binding Proteins
- Access Rights
- Free to All
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Cryo-EM structure of Csm-crRNA binary complex in type III-A CRISPR-Cas system
- Authors
- Jia, NingWang, ChongyuanEng, Edward T.Patel, Dinshaw J.
- Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of Csm-crRNA binary complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included in the deposit,...
- Subject
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CRISPR-Cas SystemsRNA-Binding Proteins
- Access Rights
- Free to All
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Cryo-EM structure of ternary Csm-crRNA-target RNA with anti-tag sequence complex in type III-A CRISPR-Cas system
- Authors
- Jia, NingWang, ChongyuanEng, Edward T.Patel, Dinshaw J.
- Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of ternary Csm-crRNA-target RNA with anti-tag sequence complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small...
- Subject
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CRISPR-Cas SystemsRNA-Binding Proteins
- Access Rights
- Free to All
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Cryo-EM structure of larger Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system
- Authors
- Jia, NingWang, ChongyuanEng, Edward T.
- Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of larger Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included...
- Subject
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CRISPR-Cas SystemsRNA-Binding Proteins
- Access Rights
- Free to All
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Cryo-EM structure of Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system
- Authors
- Jia, NingWang, ChongyuanEng, Edward T.Patel, Dinshaw J.
- Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included in...
- Subject
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CRISPR-Cas SystemsRNA-Binding Proteins
- Access Rights
- Free to All
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Crystal structure of Csm1-Csm4 subcomplex in the type III-A CRISPR-Csm interference complex
- Authors
- Jia, NingPatel, Dinshaw J.
- Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Crystal structure of Csm1-Csm4 subcomplex in the type III-A CRISPR-Csm interference complex. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included in...
- Subject
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CRISPR-Cas SystemsRNA-Binding Proteins
- Access Rights
- Free to All
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HyperTRIBE uncovers MSI2 increased RNA binding activity and differential regulation in leukemic stem cells [LSK cells]
- Authors
- Nguyen, Diu Thi ThanhLu, YuhengChoo, Zi-NingChu, Karen6 more author(s)...
- Description
Summary from the GEO: " Understanding RBPs’ molecular functions as well as their cell-type specific activity requires identification of RBPs’ direct mRNA targets. However, efforts to identify RNA targets of RNA binding proteins in stem cells have been hindered by limited cell numbers. Here we adapted the HyperTRIBE method using an RBP fused to a Drosophila RNA editing enzyme (ADAR) to allow for global...
- Subject
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LeukemiaRNA-Binding Proteins
- Access Rights
- Free to All
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HyperTRIBE uncovers MSI2 increased RNA binding activity and differential regulation in leukemic stem cells [LSC and LSK]
- Authors
- Nguyen, Diu Thi ThanhLu, YuhengChoo, Zi-NingChu, Karen6 more author(s)...
- Description
Summary from the GEO: "Understanding RBPs’ molecular functions as well as their cell-type specific activity requires identification of RBPs’ direct mRNA targets. However, efforts to identify RNA targets of RNA binding proteins in stem cells have been hindered by limited cell numbers. Here we adapted the HyperTRIBE method using an RBP fused to a Drosophila RNA editing enzyme (ADAR) to allow for global...
- Subject
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LeukemiaRNA-Binding Proteins
- Access Rights
- Free to All
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HyperTRIBE uncovers MSI2 increased RNA binding activity and differential regulation in leukemic stem cells [MOLM-13]
- Authors
- Nguyen, Diu Thi ThanhLu, YuhengChoo, Zi-NingChu, Karen6 more author(s)...
- Description
Summary from the GEO: "Understanding RBPs’ molecular functions as well as their cell-type specific activity requires identification of RBPs’ direct mRNA targets. However, efforts to identify RNA targets of RNA binding proteins in stem cells have been hindered by limited cell numbers. Here we adapt the HyperTRIBE method using an RBP fused to a Drosophila RNA editing enzyme (ADAR) to allow for global...
- Subject
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LeukemiaRNA-Binding Proteins
- Access Rights
- Free to All