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Results Found: 15
  • TCR affinity defines tumor-specific CD8 T cell differentiation and dysfunction [RNA-seq]

    Authors
    Schietinger, Andrea
    Shakiba, Mojdeh
    Betel, Doron
    Zumbo, Paul
    1 more author(s)...
    Description

    Summary from GEO: "RNA-seq analysis of tumor-specific CD8 T cells encountering tumor antigens with varying TCR/pMHC affinity." Overall design from GEO: "Transgenic antigen-specific CD8 T cells were adoptively transferred into tumor-bearing mice, isolated and flow-sorted from tumor draining lymph nodes and tumors at distinct time points post transfer for subsequent RNA-seq analysis. MCA 205 tumor...

    Subject
    Antigens, Neoplasm
    CD8-Positive T-Lymphocytes
    Cell Line, Tumor
    RNA-Seq
    Access Rights
    Free to All
  • TCR affinity defines tumor-specific CD8 T cell differentiation and dysfunction [ATAC-seq]

    Authors
    Schietinger, Andrea
    Shakiba, Mojdeh
    Zumbo, Paul
    Dunbar, Andrew
    1 more author(s)...
    Description

    Summary from GEO: "ATACseq analysis of tumor-infiltrating CD8 T cells encountering high (F6) or low (D4) affinity tumor antigen." Overall design from GEO: "Transgenic antigen-specific CD8 T cells were adoptively transferred into tumor-bearing mice, isolated and flow-sorted from tumors 14 days post-transfer for subsequent ATAC-seq analysis. MCA 205 tumor cell lines were generated to express a...

    Subject
    Antigens, Neoplasm
    CD8-Positive T-Lymphocytes
    Cell Line, Tumor
    Chromatin Immunoprecipitation Sequencing
    Access Rights
    Free to All
  • Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [RNA-Seq]

    Authors
    Rao, Anjali
    Campbell, Nathaniel
    Xavier, Joao B.
    White, Richard Mark
    1 more author(s)...
    Description

    Summary from GEO: "We report single-cell gene expression profiling of the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV under multiple in vitro and in vivo (zebrafish transplant) conditions." Overall design from GEO: "Single-cell RNA-seq of ZMEL1-PRO and ZMEL1-INV cells in one of four conditions: 1) in vitro individual culture; 2) in vitro co-culture; 3) in vivo primary tumors; and 4)...

    Subject
    Cell Line, Tumor
    Melanoma
    Neoplasm Metastasis
    RNA-Seq
    Single-Cell Gene Expression Analysis
    Access Rights
    Free to All
  • Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [3D culture]

    Authors
    Campbell, Nathaniel
    Xavier, Joao B.
    White, Richard Mark
    Description

    Summary from GEO: "We report the gene expression differences between the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV." Overall design from GEO: "RNA-seq of ZMEL1-PRO and ZMEL1-INV cells grown as clusters (3D) in Corning ultra-low attachment plates."

    Subject
    Cell Culture Techniques, Three Dimensional
    Cell Line, Tumor
    Gene Expression
    Melanoma
    Neoplasm Metastasis
    RNA-Seq
    Access Rights
    Free to All
  • Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [tfap2a/e]

    Authors
    Campbell, Nathaniel
    Xavier, Joao B.
    White, Richard Mark
    Description

    Summary from GEO: "We report how the ZMEL1-PRO cell line changes following CRISPR-Cas9 inactivation of tfap2a and tfap2e." Overall design from GEO: "RNA-seq of ZMEL1-PRO zebrafish melanoma cells following CRISPR-Cas9 with either tfap2a and tfap2e sgRNA or non-targeting control sgRNA (N=3 replicate samples each)."

    Subject
    Cell Line, Tumor
    CRISPR-Cas Systems
    Melanoma
    Neoplasm Metastasis
    RNA-Seq
    Access Rights
    Free to All
  • Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [2D culture]

    Authors
    Campbell, Nathaniel
    Xavier, Joao B.
    White, Richard Mark
    Description

    Summary from GEO: "We report the gene expression differences between the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV." Overall design from GEO: "RNA-seq of ZMEL1-PRO and ZMEL1-INV cells grown in standard adherent (2D) culture."

    Subject
    Cell Culture Techniques
    Cell Line, Tumor
    Melanoma
    Neoplasm Metastasis
    RNA-Seq
    Access Rights
    Free to All
  • Validation of dedifferentiated liposarcoma (DDLS) cell lines with the original tumors via copy number alteration analysis [sequencing]

    Authors
    Okada, Tomoyo
    Gularte-Mérida, Rodrigo
    Shen, Yawei
    Singer, Samuel
    Description

    Summary from GEO: "We validated patient's tumor-derived cell lines of liposarcoma by analysing copy number alterations using aCGH or WGS and comparing with the original tumor tissues." Overall design from GEO: "Examination of copy number alterations for 2 cell lines, one of the original tumor, one PDX-tumor and its original tumor."

    Subject
    Cell Line, Tumor
    DNA Copy Number Variations
    Liposarcoma
    Access Rights
    Free to All
  • Validation of dedifferentiated liposarcoma (DDLS) cell lines with the original tumors via copy number alteration analysis [array]

    Authors
    Okada, Tomoyo
    Gularte-Mérida, Rodrigo
    Shen, Yawei
    Singer, Samuel
    Description

    Summary from GEO: "We validated patient's tumor-derived cell lines of liposarcoma by analysing copy number alterations using aCGH or WGS and comparing with the original tumor tissues." Overall design from GEO: "Examination of copy number alterations for 2 cell lines, one of the original tumor, one PDX-tumor and its original tumor."

    Subject
    Cell Line, Tumor
    DNA Copy Number Variations
    Liposarcoma
    Access Rights
    Free to All
  • HOXA13 Cut & Run of Human Melanoma Cells

    Authors
    Weiss, Joshua M.
    Baggiolini, Arianna
    Koche, Richard Patrick
    White, Richard Mark
    Description

    Summary from GEO: "The goal of the experiment was to determine HOXA13 binding loci in human melanoma cells to infer downstream targets of a HOXA13 positional program." Overall design from GEO: "We performed Cut&Run for the melanoma cell lines SKME-1088, SKMEL-1176, and SKME-1206. For Cut&Run we used 100’000 cells per condition and it was performed as described in110. We used antibodies against...

    Subject
    Binding sites
    Cell Line, Tumor
    Genes, Homeobox
    Melanoma
    Access Rights
    Free to All
  • CD32A Immunoprecipitation in K562 Cell Membrane and Cytoplasm Fractions

    Authors
    Li, Zhuoning
    Abdel-Wahab, Omar Ibrahim
    Description

    Description from PRIDE: "Here we interrogate CD32A protein interactions in the cytoplasm and membrane fractions isolated from the human AML cell lines K562 wild-type and knockout for CD32A. Cells were fractionated prior to incubation with CD32A antibody and subsequent samples isolated using protein agarose beads."

    Subject
    Cell Line, Tumor
    Cytoplasm
    Gene Knockout Techniques
    Leukemia, Myeloid, Acute
    Access Rights
    Free to All