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TCR affinity defines tumor-specific CD8 T cell differentiation and dysfunction [RNA-seq]
- Authors
- Schietinger, AndreaShakiba, MojdehBetel, DoronZumbo, Paul1 more author(s)...
- Description
Summary from GEO: "RNA-seq analysis of tumor-specific CD8 T cells encountering tumor antigens with varying TCR/pMHC affinity." Overall design from GEO: "Transgenic antigen-specific CD8 T cells were adoptively transferred into tumor-bearing mice, isolated and flow-sorted from tumor draining lymph nodes and tumors at distinct time points post transfer for subsequent RNA-seq analysis. MCA 205 tumor...
- Subject
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Antigens, NeoplasmCD8-Positive T-LymphocytesCell Line, TumorRNA-Seq
- Access Rights
- Free to All
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TCR affinity defines tumor-specific CD8 T cell differentiation and dysfunction [ATAC-seq]
- Authors
- Schietinger, AndreaShakiba, MojdehZumbo, PaulDunbar, Andrew1 more author(s)...
- Description
Summary from GEO: "ATACseq analysis of tumor-infiltrating CD8 T cells encountering high (F6) or low (D4) affinity tumor antigen." Overall design from GEO: "Transgenic antigen-specific CD8 T cells were adoptively transferred into tumor-bearing mice, isolated and flow-sorted from tumors 14 days post-transfer for subsequent ATAC-seq analysis. MCA 205 tumor cell lines were generated to express a...
- Subject
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Antigens, NeoplasmCD8-Positive T-LymphocytesCell Line, TumorChromatin Immunoprecipitation Sequencing
- Access Rights
- Free to All
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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [RNA-Seq]
- Authors
- Rao, AnjaliCampbell, NathanielXavier, Joao B.White, Richard Mark1 more author(s)...
- Description
Summary from GEO: "We report single-cell gene expression profiling of the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV under multiple in vitro and in vivo (zebrafish transplant) conditions." Overall design from GEO: "Single-cell RNA-seq of ZMEL1-PRO and ZMEL1-INV cells in one of four conditions: 1) in vitro individual culture; 2) in vitro co-culture; 3) in vivo primary tumors; and 4)...
- Subject
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Cell Line, TumorMelanomaNeoplasm MetastasisRNA-SeqSingle-Cell Gene Expression Analysis
- Access Rights
- Free to All
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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [3D culture]
- Authors
- Campbell, NathanielXavier, Joao B.White, Richard Mark
- Description
Summary from GEO: "We report the gene expression differences between the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV." Overall design from GEO: "RNA-seq of ZMEL1-PRO and ZMEL1-INV cells grown as clusters (3D) in Corning ultra-low attachment plates."
- Subject
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Cell Culture Techniques, Three DimensionalCell Line, TumorGene ExpressionMelanomaNeoplasm MetastasisRNA-Seq
- Access Rights
- Free to All
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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [tfap2a/e]
- Authors
- Campbell, NathanielXavier, Joao B.White, Richard Mark
- Description
Summary from GEO: "We report how the ZMEL1-PRO cell line changes following CRISPR-Cas9 inactivation of tfap2a and tfap2e." Overall design from GEO: "RNA-seq of ZMEL1-PRO zebrafish melanoma cells following CRISPR-Cas9 with either tfap2a and tfap2e sgRNA or non-targeting control sgRNA (N=3 replicate samples each)."
- Subject
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Cell Line, TumorCRISPR-Cas SystemsMelanomaNeoplasm MetastasisRNA-Seq
- Access Rights
- Free to All
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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [2D culture]
- Authors
- Campbell, NathanielXavier, Joao B.White, Richard Mark
- Description
Summary from GEO: "We report the gene expression differences between the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV." Overall design from GEO: "RNA-seq of ZMEL1-PRO and ZMEL1-INV cells grown in standard adherent (2D) culture."
- Subject
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Cell Culture TechniquesCell Line, TumorMelanomaNeoplasm MetastasisRNA-Seq
- Access Rights
- Free to All
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Validation of dedifferentiated liposarcoma (DDLS) cell lines with the original tumors via copy number alteration analysis [sequencing]
- Authors
- Okada, TomoyoGularte-Mérida, RodrigoShen, YaweiSinger, Samuel
- Description
Summary from GEO: "We validated patient's tumor-derived cell lines of liposarcoma by analysing copy number alterations using aCGH or WGS and comparing with the original tumor tissues." Overall design from GEO: "Examination of copy number alterations for 2 cell lines, one of the original tumor, one PDX-tumor and its original tumor."
- Subject
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Cell Line, TumorDNA Copy Number VariationsLiposarcoma
- Access Rights
- Free to All
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Validation of dedifferentiated liposarcoma (DDLS) cell lines with the original tumors via copy number alteration analysis [array]
- Authors
- Okada, TomoyoGularte-Mérida, RodrigoShen, YaweiSinger, Samuel
- Description
Summary from GEO: "We validated patient's tumor-derived cell lines of liposarcoma by analysing copy number alterations using aCGH or WGS and comparing with the original tumor tissues." Overall design from GEO: "Examination of copy number alterations for 2 cell lines, one of the original tumor, one PDX-tumor and its original tumor."
- Subject
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Cell Line, TumorDNA Copy Number VariationsLiposarcoma
- Access Rights
- Free to All
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HOXA13 Cut & Run of Human Melanoma Cells
- Authors
- Weiss, Joshua M.Baggiolini, AriannaKoche, Richard PatrickWhite, Richard Mark
- Description
Summary from GEO: "The goal of the experiment was to determine HOXA13 binding loci in human melanoma cells to infer downstream targets of a HOXA13 positional program." Overall design from GEO: "We performed Cut&Run for the melanoma cell lines SKME-1088, SKMEL-1176, and SKME-1206. For Cut&Run we used 100’000 cells per condition and it was performed as described in110. We used antibodies against...
- Subject
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Binding sitesCell Line, TumorGenes, HomeoboxMelanoma
- Access Rights
- Free to All
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CD32A Immunoprecipitation in K562 Cell Membrane and Cytoplasm Fractions
- Authors
- Li, ZhuoningAbdel-Wahab, Omar Ibrahim
- Description
Description from PRIDE: "Here we interrogate CD32A protein interactions in the cytoplasm and membrane fractions isolated from the human AML cell lines K562 wild-type and knockout for CD32A. Cells were fractionated prior to incubation with CD32A antibody and subsequent samples isolated using protein agarose beads."
- Subject
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Cell Line, TumorCytoplasmGene Knockout TechniquesLeukemia, Myeloid, Acute
- Access Rights
- Free to All