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HEK293T cell degradome (pulse chase) time course (5h, 10, 15h) analysis upon Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)
- Authors
- Ordureau, Alban Simon GeorgesHarper, Wade J.
- Description
HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) time course experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 5h, 10h or 15h. Samples are Biotin-click, and streptavidin enriched, digested and labeled with TMTpro 16plex.
- Subject
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HEK293 CellsMass spectrometryMTOR InhibitorsProteomicsSequence Analysis, RNA
- Access Rights
- Free to All
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HEK293T cell translatome analysis upon Torin1 treatment or single amino acid withdrawal. Related to Supplementary Table 2 from An et al (2020)
- Authors
- Ordureau, Alban Simon GeorgesHarper, Wade J.
- Description
HEK293T subject to Torin1 treatment or amino acid withdraw dataset and translatome analysis was determined using Azidohomoalanine (AHA) incorportation, Biotin-click, and streptavidin enrichment. TMT 10plex labelling was used. Three genotypes, WT (24 fractions), ATG7 KO (HA-9-53-1 single shot), RB1CC1 KO (HA-9-53-2 single shot).
- Subject
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HEK293 CellsMass spectrometryMTOR InhibitorsProteomics
- Access Rights
- Free to All
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HCT116 cell proteome remodeling upon Torin1 treatment or amino acid withdraw. Related to Supplementary Table 1 from An et al (2020)
- Authors
- Ordureau, Alban Simon GeorgesHarper, Wade J.
- Description
HCT116 cells with various genotype (WT, ATCG5 KO, RB1CC1 KO), treated with Torin1 or amino acid withdraw. TMT 11 plex single shot.
- Subject
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HCT116 CellsMass spectrometryMTOR InhibitorsProteomics
- Access Rights
- Free to All
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HEK293 cell proteome remodeling upon Torin1 treatment or amino acid withdraw. Related to Supplementary Table 1 from An et al (2020)
- Authors
- Ordureau, Alban Simon GeorgesHarper, Wade J.
- Description
HEK293 cells with two genotype (WT or ATCG5 KO), treated with Torin1 or amino acid withdraw. TMT 11 plex.
- Subject
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HEK293 CellsMass spectrometryMTOR InhibitorsProteomics
- Access Rights
- Free to All
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HEK293T cell proteome remodeling upon Torin1 treatment or amino acid withdraw in WT and RPS3-Halo knock-in cells with or without NUFIP1 deletion. Related to Supplementary Table 5 from An et al (2020)
- Authors
- Ordureau, Alban Simon GeorgesHarper, Wade J.
- Description
HEK293T proteome (single shot) with knock-in or not for RPS3-Halo and Knock out or not for NUFIP1. Cells were treated or not with Torin1 or deprived of amino acids.
- Subject
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HEK293 CellsMTOR InhibitorsProteomeRNA-Binding Proteins
- Access Rights
- Free to All
