Summary from the GEO: "Our study represents a detailed molecular analysis of neuroenedocrine prostate cancer models" Overall design from the GEO: "Understanding the global methylation profile of tumor organoids and PDOXs." Abstract from the article on Synapse to further explain the study and the relating dataset: "A major hurdle in the study of rare tumors is a lack of existing preclinical models....

Summary from GEO: "Here, we showed that acute lost H3K4me3 induces rapid reduction in global transcription with progressively increase in magnitude over the time-course cooccurrence with lost all H3K4 methylations. To further determine the effects of COMPASS subunits degradation on H3K4 methylations occupancies genome-wide, we performed time-course spike-in chromatin immunoprecipitation and sequencing...

Summary from GEO "Here, we showed that acute lost H3K4me3 induces rapid reduction in global transcription with progressively increase in magnitude over the time-course cooccurrence with lost all H3K4 methylations." Overall design from GEO: "To define the primary effects of rapid INTS11 removal on transcription, we measured the synthesis of newly transcribed RNAs using a Thiol(SH)-linked alkylation...

Summary from GEO: "Here, we showed that acute lost H3K4me3 induces rapid reduction in global transcription with progressively increase in magnitude over the time-course cooccurrence with lost all H3K4 methylations. To further determine the effects of COMPASS subunits degradation on H3K4 methylations occupancies genome-wide, we performed time-course spike-in chromatin immunoprecipitation and sequencing...

Summary from GEO: "We measured the the dwell time of existed paused RNAPII at the promoter by mammalian native elongating transcript sequencing (mNET-seq). We found a median RNAPII half-life of 5.3 min at protein-coding genes in DMSO pre-treated control mESCs, whereas the RNAPII half-life at these genes significantly slowdown to 8.96 min when pre-treated with Auxin in the DPY30-mAID degron cell....

Summary from GEO: "Here, we showed that acute lost H3K4me3 induces rapid reduction in global transcription with progressively increase in magnitude over the time-course cooccurrence with lost all H3K4 methylations." Overall design from GEO: "To define the primary effects of rapid H3K4me3 removal on transcription, we measured the synthesis of newly transcribed RNAs using a Thiol(SH)-linked alkylation...

Summary from GEO: "Here, we use elongation velocity analysis showed that acute loss H3K4me3 caused a general transcriptome-wide attenuation of elongation velocity in cells." Overall design from GEO: "In order to examine and monitor the effects of H3K4me3 on newly synthesized RNA rates genome-wide from actively transcribing RNAPII, we performed the modified transient transcriptome sequencing (TTchem-seq)...

Summary from GEO: "Here, we use elongation velocity analysis showed that acute loss INTS11 caused a general transcriptome-wide attenuation of elongation velocity in cells." Overall design from GEO: "In order to examine and monitor the effects of INTS11 on newly synthesized RNA rates genome-wide from actively transcribing RNAPII, we performed the modified transient transcriptome sequencing (TTchem-seq)...

Summary from GEO: "We measured the the dwell time of existed paused RNAPII at the promoter by mammalian native elongating transcript sequencing (mNET-seq)." Overall design from GEO: "In order to examine and monitor the effects of INTS11 on the temporal stability of promoter RNAPII, we performed the mammalian native elongating transcript sequencing (mNET-seq) on INTS11-FKBP dTAG degron cells."

Trimethylation of histone H3 lysine 4 (H3K4me3) is associated with transcriptional start sites and proposed to regulate transcription initiation. However, redundant functions of the H3K4 SET1/COMPASS methyltransferase complexes complicate elucidation of the specific role of H3K4me3 in transcriptional regulation. Here, we show that acute ablation of shared subunits of the SET1/COMPASS complexes leads...