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Characterizing and classifying neuroendocrine neoplasms through microRNA sequencing and data mining
- Authors
- Nanayakkara, JinaYang, XiaojingTyryshkin, KathrinWong, Justin J.M.14 more author(s)...
- Description
From Dryad entry: "Abstract Neuroendocrine neoplasms (NENs) are clinically diverse and incompletely characterized cancers that are challenging to classify. MicroRNAs (miRNAs) are small regulatory RNAs that can be used to classify cancers. Recently, a morphology-based classification framework for evaluating NENs from different anatomic sites was proposed by experts, with the requirement of improved...
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MicroRNAsNeuroendocrine TumorsSequence Analysis, RNA
- Access Rights
- Free to All
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Frequent 4EBP1 amplification induces synthetic dependence on FGFR signaling in cancer
- Authors
- Mohan, PrathibhaPasion, JoyceLailler, Nathaliede Stanchina, Elisa6 more author(s)...
- Description
Summary from GEO: "The eIF4E translation initiation factor has oncogenic properties and concordantly, the inhibitory eIF4E-binding protein (4EBP1) is considered a tumor suppressor. The exact molecular effects of 4EBP1 activation in cancer are still unknown. Surprisingly, 4EBP1 is a target of genomic copy number gains (Chr. 8p11) in breast and lung cancer. We notice that 4EBP1 gains are genetically...
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DoxycyclineEukaryotic Initiation Factor-4EMicroRNAsRibosome ProfilingTumor Suppressor Proteins
- Access Rights
- Free to All
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14-3-3 epsilon is an intracellular component of TNFR2 receptor complex and its activation protects against osteoarthritis
- Authors
- Fu, WenyuHettinghouse, AubryannaHu, WenhuoLiu, Chuan-Ju
- Description
Summary from GEO: "In an effort to identify novel differentially-expressed genes in OA, we performed RNA-seq analysis using cartilage isolated from nonarthritic and OA patients. Total RNA was isolated from 3 samples of nonarthritic cartilage and 4 samples of OA cartilage (Kellgren-Lawrence Grade 3 or 4). Genes (900 total, 600 up-regulated, 300 down-regulated) were differentially expressed in OA vs....
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14-3-3 ProteinsGene Expression ProfilingMicroRNAsOsteoarthritisReceptors, Tumor Necrosis Factor, Type II
- Access Rights
- Free to All
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Analysis of transcriptomes in intestinal stem cells in response to Interferon gamma
- Authors
- Hanash, AlanTakashima, Shuichiro
- Description
Summary from GEO: "There is limited understanding of how immune-mediated damage impacts intestinal stem cells (ISCs). We found that stem cell compartment injury is a shared feature of both alloreactive and autoreactive intestinal immunopathology, reducing ISCs and impairing their recovery in T-cell-mediated injury models. Modeling with ex vivo epithelial cultures indicated ISC depletion and impaired...
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Epithelial CellsMicroRNAsStem CellsT-LymphocytesTranscriptome
- Access Rights
- Free to All
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Promiscuous splicing-derived hairpins are dominant substrates of tailing-mediated defense of miRNA biogenesis in mammals
- Authors
- Lee, SeungjaeJee, DavidSrivastava, SidYang, Acong7 more author(s)...
- Description
Summary from GEO: "A canonical pathway generates microRNAs (miRNAs) via stepwise cleavage of hairpin precursors by RNase III enzymes Drosha and Dicer, followed by loading of resultant ~22 nucleotide (nt) RNAs into an Argonaute (Ago) effector complex. In addition, non-canonical substrates can bypass either RNase III enzyme to yield functional small RNAs, including hundreds of intron-derived hairpins...
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HEK293 CellsK562 CellsMicroRNAsRNA, Small Interfering
- Access Rights
- Free to All
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Novel metabolic adaptation promotes enhanced macrophage efferocytosis under a limited-oxygen environment
- Authors
- Wang, Ya-TingTrzeciak, Alissa J.Rojas, Waleska SaitzSaavedra, Pedro7 more author(s)...
- Description
Summary from GEO: "Apoptotic cell clearance (efferocytosis), a process essential for organismal homeostasis, is performed by phagocytes that inhabit a wide range of environments, including physiologic hypoxia. Here, we find macrophages, the predominant tissue-resident phagocyte, display enhanced efferocytosis under chronic hypoxia, characterized by increased internalization and accelerated degradation...
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EfferocytosisHypoxiaMacrophagesMicroRNAsPentose Phosphate Pathway
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- Free to All
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Effects of Ars2 or DGCR8 siRNA on microRNA expression
- Authors
- Olejniczak, Scott HenryGruber, JoshuaDreyfuss, GideonThompson, Craig B.
- Description
Summary from GEO: "Ars2 is a component of the nuclear cap-binding complex that is required for cellular proliferation and contributes to microRNA biogenesis. Arrays were performed to determine the repertoire of microRNAs that change following knock-down of Ars2. Knock-down of DGCR8 was also performed to determine which microRNAs were expressed and had half-lives short enough to significantly decrease...
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Gene ExpressionGene Knockdown TechniquesHeLa CellsMicroRNAsRNA-Binding Proteins
- Access Rights
- Free to All
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Effects of Ars2 or DGCR8 siRNA on gene expression
- Authors
- Olejniczak, Scott HenryGruber, JoshuaDreyfuss, GideonThompson, Craig B.
- Description
Summary from GEO: "Ars2 is a component of the nuclear cap-binding complex that is required for cellular proliferation and contributes to microRNA biogenesis. Arrays were performed to determine the repertoire of genes that change following knock-down of Ars2. Knock-down of DGCR8 was also performed to determine which changes in Ars2 knock-down cells resulted from defects in microRNA expression."...
- Subject
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Gene ExpressionGene Knockdown TechniquesHeLa CellsMicroRNAsRNA-Binding Proteins
- Access Rights
- Free to All