Summary from the GEO: "An inadequate supply of amino acids leads to accumulation of uncharged tRNAs, which can bind and activate GCN2 kinase to reduce translation. Here, we show that glutamine-specific tRNAs selectively become uncharged when extracellular amino acid availability is compromised. In contrast, all other tRNAs retain charging of their cognate amino acids in a manner that is dependent...

This work aimed to improve sensitivity of targeted detection by orbitrap mass spectrometers. Co-isolation of contaminant ions was identified as the major factor limiting sensitivity, and LOD of both PRM and accumulated precursor ion scanning (AIM) was improved by increased chromatographic resolution.

Recent development of mass spectrometer cleavable protein cross-linkers and algorithms for their spectral identification now permits large-scale cross-linking mass spectrometry (XL-MS). Here, we optimized the use of cleavable disuccinimidyl sulfoxide (DSSO) cross-linker for labeling native protein complexes in live human cells. We applied a generalized linear mixture model to calibrate cross-link...

This dataset was generated to parametrize and benchmark software for unbiased discovery of post-translationally modified peptides.

This dataset was generated to model discovery of uncommon and non-canonical PTM by agnostic analysis. Synthetic DFSAFILVEFCR peptides were obtained in three chemoforms, all with carbamidomethylated C12: 1) with unmodified phenylalanines, 2) with fluoro-F2, and 3) with fluoro-F11. Peptide composition and chemical modifications were confirmed using mass spectrometry. Peptides were analyzed in neat solvent...

This dataset was generated to confirm that +130 and +146 Da adducts observed in LPS-stimulated macrophages were produced by the itaconate metabolite. To this end, model proteins (bovine serum albumin and human KEAP1) were reacted in vitro with itaconate, and the correspondent adducts were analyzed by LC-MSMS

RNAse J, HVO_2724 from halophilic archaeon to identify the proteins it associates with

Purification of RecJ3/4-RNase J complex from Haloferax volcanii