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Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis (CHX experiments)
- Authors
- Garg, AngadShuman, Stewart H.Schwer, Beate
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of fission yeast cells (Schizosaccharomyces pombe) at various times after phosphate starvation (2, 4, and 8 HR) either with 100 µg/ml cycloheximide or without drug contrasting to the cells grown in phosphate replete conditions (0HR) in the absence of the drug." Overall design from GEO: "6 test samples (cycloheximide or no drug...
- Subject
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CycloheximidePhosphates/metabolismRNA-SeqSchizosaccharomycesSchizosaccharomyces pombe Proteins
- Access Rights
- Free to All
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Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis
- Authors
- Garg, AngadShuman, Stewart H.
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of fission yeast (Schizosaccharomyces pombe) strains prior to phosphate starvation (0 HR) and at various times after phosphate starvation (4, 8, 12, 24, 36, and 48 HR) contrasting to the WT fission yeast S. pombe cells grown in phosphate replete conditions (WT 0HR)." Overall design from GEO: "13 test samples and 1 control sample...
- Subject
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Phosphates/metabolismRNA-SeqSchizosaccharomycesSchizosaccharomyces pombe Proteins
- Access Rights
- Free to All
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SPX domain proteins are agents of inositol pyrophosphate toxicosis in fission yeast
- Authors
- Garg, AngadSchwer, BeateShuman, Stewart H.
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of spx1∆ mutant contrasting to the WT fission yeast S. pombe." Overall design from GEO: "1 test sample and 1 control sample was processed (each sample has 3 replicate experiments). WT samples cultured at the same time and sequenced in the same run were previously deposited in GEO dataset GSE164616."
- Subject
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Gene ExpressionNuclear ProteinsRNA-SeqSchizosaccharomyces
- Access Rights
- Free to All
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Inositol pyrophosphate dynamics impact fission yeast phosphate homeostasis via modulation of RNA 3' processing and transcription termination
- Authors
- Garg, AngadSchwer, BeateShuman, Stewart H.
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of the Inositol pyrophosphate phosphatase mutant aps1∆ and Inositol pyrophsophate kinase mutant asp1-D333A contrasting to the WT in the fission yeast S. pombe." Overall design from GEO: "2 test samples (mutants) were analyzed; 1 WT control sample was processed (each sample has 3 replicate experiments)."
- Subject
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Gene ExpressionInositol PhosphatesPyrophosphatasesRNA-Seq
- Access Rights
- Free to All
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Genetic interactions and transcriptomics implicate fission yeast CTD prolyl isomerase Pin1 as an agent of RNA 3' processing and transcription termination that functions via its effects on CTD phosphatase Ssu72
- Authors
- Garg, AngadSanchez, Ana M.Schwer, BeateShuman, Stewart H.
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of the peptidyl-prolyl isomerase Pin1 mutant pin1∆ contrasting to the WT in the fission yeast S. pombe." Overall design from GEO: "1 test sample (mutant) was analyzed; 1 WT control sample, sequenced at the same time, was previously deposited in GEO dataset GSE131237 (each sample has 3 replicate experiments)."
- Subject
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Gene ExpressionNIMA-Interacting Peptidylprolyl IsomerasePyrophosphatasesRNA-SeqSchizosaccharomyces
- Access Rights
- Free to All
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Fission yeast poly(A) polymerase active site mutation Y86D alleviates the rad24 Δ asp1-H397A synthetic growth defect and up-regulates mRNAs targeted by MTREC and Mmi1
- Authors
- Garg, AngadShuman, Stewart H.Schwer, Beate
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of the pla1-Y86D mutant contrasting to the isogenic wild-type fission yeast (Schizosaccharomyces pombe)." Overall design from GEO: "1 test samples and 1 control sample was processed (each sample has 3 replicate experiments)."
- Subject
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Gene ExpressionmRNA Cleavage and Polyadenylation FactorsRNA-SeqSchizosaccharomyces
- Access Rights
- Free to All
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Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis (mass spectrometry proteomics data)
- Authors
- Garg, AngadShuman, Stewart H.
- Description
Inorganic phosphate is an essential nutrient acquired by cells from their environment. Here we characterize the adaptative responses of fission yeast to chronic phosphate starvation, during which cells enter a state of quiescence, initially fully reversible upon replenishing phosphate after 2 days but resulting in gradual loss of viability during 4 weeks of starvation. Time-resolved analyses of changes...
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Liquid Chromatography-Mass SpectrometryMaf Transcription FactorsPhosphates/metabolismProtease InhibitorsProteomeRNA, Transfer
- Access Rights
- Free to All
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Duf89 abets lncRNA control of fission yeast phosphate homeostasis via its antagonism of precocious lncRNA transcription termination
- Authors
- Garg, AngadSanchez, Ana M.Shuman, Stewart H.Schwer, Beate
- Description
Summary from GEO: "This RNA-Seq analysis compares gene expression of the Duf89 null mutant (duf89∆) contrasting to the WT in the fission yeast S. pombe"
- Subject
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Gene ExpressionRNA-SeqSchizosaccharomyces/genetics
- Access Rights
- Free to All